Be or oI ae 
1897] BRIEFER ARTICLES 41 
having been developed since entering the oosphere. In the case of Pa 
silvestris, Dixon has observed that the tube nucleus and the stalk-cell 
nucleus may accompany the two male cells into 
the oosphere, but in this case no trace of these 
sterile nuclei could be found, and before the 
tube began to enter the oosphere they had given 
evidence of the beginning of disorganization. 
The most remarkable feature of the section, 
however, is the bulging of the female nucleus (F) 
towards the larger and nearer male nucleus (mM). 
Mr. Schaffner has observed‘ a similar bulging of 
the oosphere nucleus in Asma, but in that case 
the whole side of the nucleus appeared to be 
drawn out, while in the case before us there is F's. be othoas — with 
ngle suspenso 
only a papilla-like protuberance. 
Figure 2 is contributed by Mr. Charles J. Chamberlain, and is a 
fitting supplement to the stage found by Mr. Schaffner, although it 
was obtained from another species, P. Zaricio, the common Austrian 
pine of the parks. The male (m) and female (F) nuclei are in the 
initial stage of fusion, the protuberance of the latter having decidedly 
indented the former. In 2a Mr. Chamberlain has outlined t 
embryo-sac in order to locate the pairing nuclei, which are nearer the 
micropylar end. It will be observed, therefore, that the male nucleus 
is not upon the side of its entrance, a shift in position which may be 
common, or it may be the accident of the section. The male nucleus 
also has increased in size until it approximates that of the female 
nucleus, an increase that seems to begin in the case of one of the two 
: oe nuclei when they enter the oosphere, ; as shown in per. In each 
cleus the — (x) ovis broken ob saa cnecmeiees gl lobules. fed 
ino one © continaoss piece, the free ends being due to cation since ce the 
f the series. This state was discov- ie 
A a 
ag 
ered before that represented by jig. fy and We sexual nature of Se : 
two nuclei was much in doubt. The micropyla 
