42 BOTANICAL GAZETTE [JANUARY 
and an attempt was made to restain with cyanin-erythrosin, but the 
nuclei still stained alike. Dr. Watasé’s researches on the sexual 
nuclei of animals show that at the moment of fusion the nuclei stain 
alike, while before fusion the male nucleus is cyanophilous and the 
female nucleus erythrophilous. However, the present attempt at 
sexual staining proves nothing, as the sections threatened to wash off, 
and consequently the staining was not prolonged enough to become 
decisive. 
figure 3 is contributed by Mr. John G. Coulter, and represents a 
young embryo of Pinus Laricio that has developed at the end of two, 
and probably four, suspensors. The general statement that in Pinus 
each of the four independent suspensors develops an embryo breaks 
down in this species. The statement usually runs that Picea excelsa is 
the single exception among the Adzetine. In Pinus Laricio, however, 
the greatest variety was observed ; sometimes an embryo to each sus- 
pensor ; oftener an embryo to two or four suspensors, as in the figure; 
and in one case two embryos to a single suspensor, as shown in the 
accompanying cut, furnished by Mr. Schaffner. In the last case the 
primary segmentation was evidently longitudinal, the two resulting 
cells for some reason became physiologically dissociated, and each one 
of them proceeded independently to form an embryo. 
Figure gis contributed by Mr. W. D. Merrell, and represents the — 
first segmentation of an embryo of Pinus Banksiana. In this species 
it seems to be the rule for the first one or two segmentations to be 
transverse. Afterwards longitudinal divisions appear, beginning with 
the basal cell and including the apical cell. In P. Laricio the primary 
segmentation i is also usually transverse, the only exception noted being 
that represented in the text cut, and there is that general freedom 
som any fixed order in the subsequent segmentations which Stras- 
burger figures for Thuja. Nothing that could be regarded a Gee 
true apical cell was Observed in any case, for though the form of ano 
apical cell was simulated occasionally, i its subsequent history showed . : 
that it was a resemblance in form and not in fact, ~~ it never cut off — 
successive oblique segments, or even one. ow 
spite cs is sous eg! Mr. Oo. w. Caldwell, eee reprevent the , 
Lar “has passed through the 
