156 BOTANICAL GAZETTE | MARCH 
nect such stages as fig. 76 and fig. 28, so as to account fora 
macrospore without antipodals, was unsatisfactory on account 
of the numerous instances in which nuclei were fusing to form 
the primary endosperm nucleus. A prolonged search revealed 
the missing antipodal cells (figs. 26, 27). A careful exami- 
nation of about five hundred macrospores yielded six with 
indisputable antipodals and, since they are known to exist, other 
preparations show what may be reasonably interpreted as their 
disorganized remains. The antipodals are small, three in num- 
ber, and are situated at the extreme chalazal end of the macro- 
spore. In fg. 3z the three cells marked a may be antipodals, 
for there does not appear to be any trace of a pollen tube or 
other evidence of fertilization, and the nucleus marked e looks 
like the primary endosperm nucleus rather than an endosperm 
nucleus resulting from its division. A somewhat similar condi- 
tion is shown in fig. 33, but in this case there is a pollen tube 
already within the macrospore. If in either or both of these 
cases the cells marked a are cells of the endosperm, the primary 
endosperm nucleus has divided very early, and the appearance 
of the cells is unusual. It may be possible that the group of 
three cells has arisen from the division of ‘the lower polar 
nucleus without any fusion with the upper one having taken 
place. If the nuclei belong to the endosperm, these two cases 
are the only ones observed in which there were more than two 
nuclei in the endosperm before the first division of the oospore. 
In the the fusion of the polar nuclei to form the primary 
endosperm nucleus, details were not worked out, as Salix is not 
a favorable form. It may be noted merely that the fusion seems 
to be complete, even the nucleoli fusing to form one large 
nucleolus. A dense strand of protoplasm usually extends from 
the primary endosperm nucleus to the oosphere. 
A few of the cells surrounding the chalazal end of the sac 
are very ne boa ce an probably bites the influence of the 
_antipodal cells. 
It is very common to find the whole egg apparatus bursting 
through the apex of the nucellus into the micropyle. The 
