1897 | LIFE HISTORY OF LILIUM PHILADELPHICUM Al5 
micropyle, the encroachment of the enlarging macrospore is at 
first chiefly upon the tissues beneath. 
This enlargement is the first step in the ‘germination of the 
macrospore,” and when the sac has become considerably elon- 
gated the first nuclear division occurs near the center, the axis of 
the spindle being longitudinal (jig. 2), and the daughter nuclei 
passing to their polar positions. A more detailed view of the 
antipodal end of this first spindle, at an earlier stage, while the 
chromosomes are still distinct, is given in fg. 3, showing the 
usual transient cytoplasmic radiations about the chromosome 
Sroup, and the definite relation of the larger ones to the micro- 
nucleoli. The cytological phenomena connected with this divi- 
sion, known as the ‘reduction division,” form the subject of Mr. 
Schaffner’s paper. With such an abbreviated history as that of 
the Macrospore of Lilium the division representing the reduction 
division is evident, but in most angiosperms the place of this 
Special division in the history of the macrospore is not so clear. 
Immediately after the placing of the two nuclei the second 
divisions occur ( jigs. 4-8), the micropylar spindle being trans- 
verse, the antipodal one longitudinal. In figs. g and 5 the 
reduction number of chromosomes is apparent in the micro- 
Pylar spindle, while a largely increased chromatin mass is 
@pparent in the antipodal spindle. Very soon the resulting 
nuclei shift their positions more or less (figs. 7, 74, 8), so that 
the directions of the two spindles are lost. The persistence of 
the spindle fibers (figs. 7, 72) is a common phenomenon in the 
embryo sac divisions, and often helps to indicate the shifting of 
the freed nuclei. | | 
In this second division certain phenomena were noted by 
= ‘Miss Merriman which deserve mention. The occasional occur- 
_ fence of multipolar spindles in Lilium is well known, and figs. 9 
~42 may be taken to represent them. As these spindles are 
_ 4Ssociated with exceptional conditions of the chromatin band, 
4nd occurred in a single ovary, they suggest a very unusual and 
_ Possibly a pathological condition. With the claims made for — 
the relation between the multipolar spindle and the bipolar : 
