450 BOTANICAL GAZETTE [JUNE 
Fic. 9. Thin section of a macrospore with nucleus containing double rows 
of chromatin bands. The nucleolus shows a number of small vacuoles. 
Anilin-safranin, gentian-violet. 1200. 
Fig. ga. A single thread of the double chromatin band showing the 
arrangement of the chromatin granules on the linin thread. 22 
Fig. 10. Nucleus in which the double threads of the chromatin band are 
beginning to twist on each other. Anilin-safranin. gentian-violet. 1200. 
Fig. 10a. A double twisted chromatin band crossed bya single one. The 
other part was very likely cut away. 2250 
Fic. 11. Section of a nucleus in which the double twisted chromatin band 
has twisted into twelve loops with the heads toward the nuclear membrane. 
Anilin-safranin, gentian-violet. 1200. 
Fic. 11a. Adjoining section of the same nucleus. X 1200. 
Fic. 116. Two loops from fig. 77. X 2250. 
Fig. 12. Chromatin loop a little later than fg. 77. The two linin threads 
have twisted more closely together, presenting more nearly the appearance of 
a single band. Anilin-safranin, gentian-violet. xX 2250. 
FiG. 13. Thin section of macrospore showing chromosomes immediately 
after the breaking up of the chromatin band into the twelve chromosomes. 
The nuclear membrane has almost disappeared. ssieuemmcanes s haematoxylin, 
erythrosin. X 1200. 
Fic. 14. Section of macrospore showing the double nature of the chromatin 
loops, a large vacuolate nucleolus, and three micronucleoli, one of which has 
art beyond the nuclear limits. Anilin-safranin, gentian-violet. x 1200. 
G. 15. Section of macrospore showing fine cytoplasmic radiations 
Gee outward from the nucleus. In the nuclear area is one large nucle- 
olus: and numerous micronucleoli. Some of the micronucleoli have . traveled 
far out into the cytoplasm. Anilin-safranin, genatin-violet. X 1200. 
Fig. 16. Nucleolus with vacuolate bodies ; just before the breaking up of 
the chromatin band. Anilin-safranin, gentian-violet. 2250. 
Fig. 4g. 3 Nucleolus with vacuolate bodies. Anilin-safranin,  cenapetingeal 
x 225 Me 
; 1G. 18. Nucleolus with ll rical vacuolate bodies. A small nuc' 
. et lying outside « of the nuclear panties: Anilin-safranin, cena 
X 2250. 
Fig. 19. Wie-icslies with one of the vacuolate iesiies “apparently being 
extruded. Anilin-safranin, gentian-violet. x 2250. : 
Fi. 20. Outline of a nucleus, comepres one large vacuolate —— 
a micronucleolus within the nuclear area, a small vacuolate nucleolus ow 
spon ——— — oe x = : 
