452 BOTANICAL GAZETTE [JUNE 
Fic. 35. Chromosome from mother star, showing mode of division which 
appears to be an untwisting of the chromatin loop. Anilin-safranin, gentian- 
violet. X 2250. 
1G. 36. Macrospore, with nucleus in the mother star stage with numer- 
ous micronucleoli in the surrounding cytoplasm, some of which are of a larger 
size than usual, The chromosomes are ready to be pulled apart. Anilin- 
safranin, gentian-violet. X 1200. 
Fic. 36a. Pole of the spindle with centrosphere containing a double cen- 
trosome. X 2250. 
Fic. 37. Macrospore, with nucleus in the loose daughter skein stage. 
The numerous micronucleoli in the cytoplasm appear to be in close connec- 
tion with cytoplasmic radiations surrounding the daughter nuclei. Iron- 
alum, haematoxylin, erythrosin. X I200. 
Fic. 38. Close daughter skein stage with numerous micronucleoli in the 
surrounding cytoplasm, cytoplasmic radiations, and fine centrospheres at one 
pole. Anilin-safranin, gentian-violet. > 1200. 
FIG. 39. Close daughter skein stage with micronucleoli in cytoplasm and 
cytoplasmic radiations. Delafield’s haematoxylin. X 1200. 
Fig. 40. Close daughter skein with most of the micronucleoli inside of 
the nuclear area. Anilin-safranin, gentian-violet. X 1200. 
Fig. 41. Close daughter skein with most of the micronucleoli inside of 
nuclear area and the remaining ones crowded around the two nuclei. Anilin-_ 
safranin, gentian-violet. 1200 
Fig. 42. Two-celled ed sac. Only a few micronucleoli remaining in 
the cytoplasm. Anilin-safranin, gentian-violet.  I200. 
FIG. 43. Two-celled embryo sac. All the micronucleoli have entered the 
daughter nuclei, which at this stage have well-defined nuclear membranes. 
Tron-alum, haematoxylin, orange G. 1200. 
| Fic. 44. Section of two-celled embryo sac, showing nucleus in the daugh- 
_ ter star stage. Two centrospheres appear at one pole and numerous micro- 
nucleoli i in the cytoplasm. Anilin-safranin, gentian-violet. X 1200. 
iF 1G. = 45 Embryo sac with well marked cytoplasmic radiations, and the 
oli crowded around the daughter nuclei. _ The remains of the first 
Fie. 46. Embryo sac with, the two nuclei in the close daughter skein 
Numerous micronucieoli in the ee | °y fag ee ag 
‘sac wa all the atte in the resti e 
= li. No —— li are left inte 
: » gentian-violet. ‘X 1200, — 
