1902] THE PARASITISM OF BOTRYTIS CINEREA 429 
TANNIN. 
The effect of the growth of Botrytis upon commercial tannin 
was quite closely followed. It has already been mentioned that 
_ the liquid gradually became of. a darker color and finally nearly 
black. The same effect could be shown by mixing a 2 per cent. 
: solution of tannin in a flask of gelatine. A flocculent precipitate 
_ occurred, giving the hardened gelatine a white color. When 
_ inoculated with Botrytis this color began to disappear about the 
point of inoculation and turn brown. Gradually the change 
spread, keeping pace with the growth of the organism, until 
| finally the whole mass of gelatine to a considerable depth had 
thisdark color. This is an excellent method for showing this 
--Teaction, 
_ Asolution of tannin in the stock solution was divided into 
© portions and one inoculated with Botrytis. After the end 
one week the latter was dark brown in color, while the orig- 
inal portion was still nearly colorless as at first. Both gave a 
deep purple precipitate with ferric chloride. With calcium | a 
hydrate the original solution gave a white precipitate, changing So 
lilac (tannin reaction. ) That upon which Botrytis had grown» 2 
heavy precipitate, the color of which could not be clearly - 
disti Suished on account of the dark color of the liquid. Anoth 
mof the Botrytis liquid was agitated _ ether 
ve a brown precipitate rapidly darkening (gall Cag 
Solution of gelatine gave with the original tannin solu 
white precipitate. With the culture fluid no pr 
: _ After aed an excess 3 of sig t é oth 
