THE PARASITISM OF BOTRYTIS CINEREA 433 
difference in appearance was that the acid had a bleaching effect 
on the tissue, while that in the extract became darker colored. 
Microscopically some similarities and some differences were 
The cells in the acid were strongly plasmolyzed and 
In collapsing they pulled apart from one another, so 
even in the 0.125 per cent. solution the tissue was almost as 
pletely macerated as in the Botrytis extract. It may be 
sated conservatively that in a 0.125 per cent. solution of oxalic 
the death of the cells and consequent softening of the tissue — 
, fully as marked as in an average Botrytis extract. The cell 
ll was somewhat swollen in the acid, but not in the extract. The 
gration of the protoplasm was more marked in the extract. 
ary found in a sample of Sclerotinia extract 0.319 per cat 
ic acid. The writer has found over 2 pet cent. in the 
Fextracts from old cultures to whieh sugar ROU eee 
ntly supplied. It can therefore scarcely be doubted that 
marked effect 
BAG 
: tissue, whether or not the liquid had 
a reasonable objection to judging t the ¢ 
the fungus by those of such extracts, that oT 
conidia or filaments rapidly advancing” into new ae 
om nts of oxalic acid could be — Lear 
f 0.01 and 0.05 per cent. — ; 48 
on vegetable tissue studied as before. 
in these 
