6 BOTANICAL GAZETTE [janttary 



While these events are going on within the nucleus, there are 



J poles outside. The kinoplasmic cap 



formed kinoDlasmic 



fibers 



running in a longitudinal line, one end focusing at one pole, and the 

 other end diverging toward the nucleus, reaching nearly to the nuclear 

 membrane or falling short of it. Since no change ever occurs in the 



me 



ment of the cytoplasm where the cone arises. The whole cytoplasm, 

 except where the kinoplasmic caps arise, shows at this time a fine 

 fibrillar structure. Now the nuclear membrane begins to disappear 

 at the basal region of the kinoplasmic caps, the rest of it still clearly 

 limiting the nuclear contents froni the cytoplasm outside. There 

 seemed to be no regularity in the course of the spirem until this stage. 

 The entangled spirem was distributed throughout the cavity, the 

 bent parts of the loops being directed toward the membrane ; but 

 when the nuclear membrane begins to disappear near the kinoplasmic 



caps, some parts of the spirem 

 the caps. 



me 



segmentation of the spirem 



of slender 



chromosomes, and the intrusion of spindle fibers which become 

 attached to the chromosomes at their ends (fig. 2). At this time there 

 is first observed the longitudmal splitting of the chromosomes, the 

 two parts resulting from the splitting always remaining closely asso- 

 ciated. Tlie chromosomes, in general, are directed along the axis of 



poles are 



les ; one end reaches near the pole, and about the equatorial 

 the other end, to which spindle fibers from the two opposite 

 attached; so that the distance of the attached ends from 



become 



poles is approximately the length of two chromosomes. This 



ondition shown in fig. j, where the chromosomes 

 ved. The line associating the tu chromosomes 



from 



chromosomes which were so slender and peculiarly curved as : 



above, from the first segmentation to this period, now begin to sh 

 the equatorial ends, to which the spindle fibers from the two pol 



attached, remain stationaiy-, while the ends dkected to the poles 



toward the equator as the chromosomes shorten. Tn \he 



The 



course 



