32 • BOTANICAL GAZETTE [january 



The chromatin next becomes densely massed around the nucleo- 

 lus, and the irregular clumps elon^rate more or less into rods. So 



come 



margin of the somewhat 



rounded mass can a threadlike appearance of the chromatin be 

 observed. The chromatin is now gathered together at one side of 



gradually emerges from the center and 



(figs 



to one side of the chromatin. When it has completely emerged its 

 staining capacity is seen to have diminished (figs. g-12). In some 

 cases a bipartite arrangement of the chromatin is observable {figs. 



•) 



m the cells at one end of the anther 



begins to lose its power to take the stain uniformly 

 of dark clumps lying in a paler matrix is produced. The'nucleolus 

 is also usually vacuolated {jigs. 7 j, i^^ 16). The dark bodies approach 

 the nucleolus and it seems to sink into or to be surrounded by the paler 

 area Within its substance is frequently seen material staming 

 deeply like the bodies without; often this forms a partial ring around 

 a vacuole {fig. 16). The nucleolus now becomes weU iiUed with 

 darkly staining material, whHe the substance without, which takes a 

 similar stam, decreases in amount; it finally disappears entirely. 

 At the same time the nucleolus has become entirely black with saf ranin 

 red, and Kes whoUy within the paler substance {figs. 17-ig). This 

 condition was observed in S. purpurea and in 5. jlavaX purpurea. 

 1 he other species did not present suitable material for this stage. 



The 1mm next begins to extend toward the circumference of the 

 nucleus and to be converted graduaUy into a network {figs. 20-22). 

 iJark granules, purple with safranin and malachite green, now appear 

 m the net^-ork, and at the same time the nucleolus again shows 



vacuoles {figs. 2j, 24). It seems to go througu m a reNersc oruer u. 



former senes of changes, dark material within the nulceolus often 

 surroulidmg a central vacuole {fig. 23). Sometmies the nucleolus 

 presents the appearance shown in figs. 23a and 2j6. At one focus 

 hree darkly stammg spheres can be seen, whereas at a slightly higher 

 level these spheres are replaced by vacuoles. In one case a body 

 closely lesembhng the nucleolus was obsened Iving fust outside the 



to 



