igoS] 



HASSELBRING— ASSIMILATION OF PENICILLIUM 



183 



'/ 



culture 



mineral 



tables. The actual quantity as well as the concentration of the 



flask 



the 



giving the yields for each set. 



1^ 



TABLE V 



Numbeis 



Amount of CaHsKSO* 



Tield 





o.82*i^ ( = 0.1 GM. per 1.) 

 1 . 64:^^ (=0.2 GM. per L) 



none 

 none 



TABLE VI 



Number 



0.82^ C.H5KSO4 

 (-=0.1 GM. perl.) 



0.69^ CHjOH 

 (=0.3 GM, perl.) 



I 



3 



4 



5 



152* 



184 



207 

 219 

 219 



0.82^ 



CHsKSO* 



0.41^ CHsKSO* 

 (=0.05 GM. per I.) 



o.69^"'C.HjOH 



0.41* 

 CaH5KS04 



None 



None 

 None 

 None 

 None 



Check 



CHsOH 



200 

 210 

 2x4 

 228 

 238 



None 

 None 

 None 

 None 

 None 



193 



* Contaminated. 



TABLE VII 



Numbers 



CaHsNOa* 



Yield 



r-io 

 11-20 



♦The concentration of the ethyl nitrate is not stated, since only a part of the amounts givtn 

 into sohition in 50" of the culture fluid. 



TABLE VIII 



'■«•.- 





Number 



o.bg^CjasOH 



(=0.3 GM. perl.) 



0.46^ CHjNOa 

 0.69^ C^sOH 



2 



3 

 4 



S 



I 



! 



103 



52 



106 



56 



1X2 



6s 



"5 



72 



126 



89 



i 



1 

 1 



C2ieck. 



o.e^"' C.H5OH 



No organic 



matter 



(& 1X7 



94 118 



3 



112 141 





113 ^3 



116 140 





The cultures of potassium ethyl sulfate {table V) had a barely 

 visible film on the surface, similar to cultures to which no organic 

 matter had been added. Microscopic examination showed that prac- 



