234 



BOTANICAL GAZETTE 



[APRIL 



be more readily saponified by the lipase, A 0.0125 per cent, solution 

 of the enzyme yielded from o. 10^^ of butyrate enough acid to require 



0.20 



cc 



/ 



The same concentration of 



actmg for one hour longer at the same temperature 



/ 



A 



me 



740 KOH 



mi 



butyrate is the less stable in the presence of the enzymcj but it maj 



more 



case than in the other. The concentration of the enzyme in most of 



ME 



Potassium and sodium in contemporaneous test 



POTASSIUM 



m 



Water^ 



32 

 16 



8 



4 

 2 



I 



Control^ 



Enzyme 



8 



In crease 8 



o 

 o 

 o 

 o 

 o 

 o 



07 



06 



06 

 06 

 06 

 06 

 06 



0.25 



0.20 

 o. 19 

 0.16 



0.13 



0-13 



O.IO 



0.18 



0.14 



0-13 



o. 10 

 0.07 



0.07 

 0.04 



SODIUM 



32 



8 



4 



2 



0.07 

 0.07 

 0.07 

 0.07 



0.07 



O.OT 



0.20 

 0.17 



0.13 



O.IO 



0. 10 



0.13 



O.IO 



0.08 



0.06 



0.03 

 0.03 



cnz\ 



Enzyme 0.05 per cent. Incubation 5 hours at 40 C. 



^ the column headed "w" are given the various concentrations of the toxic 

 fractions of molecular strength. In this column the word *' Water" means 

 ■ of water were used instead of the toxic solution in order to ascertain the un- 

 .^ , ne in 0.05 per cent, strength. In the column beaded 



Control" are given the figures for the final acidity (cc. m/40 KOH) of the boil^ 

 preparations containing the toxic salt in the concentration indicated by the correspond- 

 ing figure in the column headed "w." The column headed "Enzvme" shows the 

 final acidity of the unboiled preparations corresponding to the controls. The column 

 headed ''Increase- shows the difference in acidity between the controls and the un- 

 boiled preparations and expresses the amount of saponification allowed by the con- 

 c^axioa of the toxic solution indicated by the corresponding figures in the column 



