222 BOTANICAL GAZETTE [mauch 



• At first the cytoplasm of the body cell seems homogeneous, 

 without any vacuoles or conspicuous granules (fig. id). The 



um 



hematoxylin. Most of the chromatin is in the form of deeply 

 staining granules. 



In a short time it is noticed that the nucleus no longer stains 

 sharply, the reticulum appearing very faint, and even the nucleolus 

 and larger chromatin granules taking scarcely any stain. But while 

 these changes are taking place within the nucleus, many granules, 

 staining sharply with iron alum hematoxylin and apparently identical 

 with the chromatin granules, appear in the cytoplasm. For the sake 

 of reference we may call them the black granules {bg, figs. 13, 14)- 

 I believe that they have come from the nucleus. Whether the granules 

 pass through the membrane bodily, or become dissolved and pass 

 through by osmosis, might be a question. Living chromatin is 

 semi-fluid and the nuclear membrane at this time is extremely thin. 

 If the nuclear membrane is formed by the condensation of cytoplasm 

 about the nuclear vacuole, the "breaking-down" of the membrane 

 in the prophase of mitosis may be merely the return of the condensed 

 cytoplasm to the ordinary alveolar condition ; and as this condition 

 approaches, but while the membrane is still recognizable, it is reason- 

 able to suppose that particles may pass from the nucleus to the 

 cytoplasm without becoming soluble. The black granules might 



_,_, in this way. There is little 



doubt that chromatin is more or less soluble. In solution, the 



asm 



hav 



mosis through a membrane with sucn 

 mierht imagine the nuclear membrane to 



e. 



The black granules are very small at first and an 

 near the nucleus. They increase in size by imbibin 



numerou- 



granule 



; llicle 



inclosing a liquid. As the pellicle stretches, granules pass 



atery 



•lin 



gradually changes from black to gray. For reference, these globus 

 may be called gray bodies (gb, figs. 14, if) . Both the black granute 

 and gray bodies are found not only in the body cell, but also in ^ e 

 stalk and prothallial cells, and even in the cells of the nucellus. 



