34^ 



JULIAN MAST WOLFSOHN. 



water the eggs would become mature, I subjected the eggs to 

 the following solutions : 50 c.c. sea water, plus 0.4 c.c. ; 0.7 

 c.c; 1.0 c.c; 1.5 c.c. 1/10 11 NaHO for 1, 1.5, 2 and 3 hours. 

 After each period the eggs were transferred to normal sea water 



Oh OT-/OS2 



Fig. 1. — Immature eggs. 



Fig. 2. — Mature eggs. 



and sperm added about fifteen minutes later. At the end of two 

 hours, maturation in most of the cases, was completed. Eighteen 

 hours later the following results were obtained : 



Table I. 



From the table we see that the greatest number of the eggs 

 become mature and form larvse when 1.5 c.c. njio NaHO are 

 added to 50 c.c. sea water, and the eggs subjected to this mix- 



