262 



LEIGH HOADLEY. 



Table Ic. 



Eggs 9 B. 5 : 10 



Viability tests. Aug. 12, '22. 5:27 P.M. Temp. 21 C. 

 P.M. Sperm fresh. Insemination 1 : 25 : 7.5. Solutions: 



A, 1 : 1,500,000 AuCU. 



B, 1 : 375,000 AuCU. 



Li lie ('21) found that there are three ways in which egg 

 when placed in the membrane-inhibiting concentration of CuCl 2 

 can be made to fertilize and produce membranes. Two of these 

 are by means of protective agents. In the first case he used 

 egg sea-water of high agglutinating power. In the second, he 

 used a solution of gelatine or of gum arabic. The third method 

 is by the use of an excess of sperm. A test was made of the 

 effect of increased sperm concentrations on gold inhibition with 

 the results shown in Table Id. A concentration of AuCl 3 of 



Drops 1 : 25 

 Sperm. 



Effect of increased sperm concentration. Aug. 7, 1922. 10 : 38 A.M. Temp. 

 25 C. Eggs 9 : 50 A.M. Sperm fresh. AuCh 1 : 750,000. 



I : 750,000 was used in this experiment. Such a concentration 



has a p.H. of 8.0 and has no visible effect on the spermatozoa. 



2. Copper Chloride. — Lillie ('21) investigated the effect of 





