THE BEHAVIOR OF CALCIUM PHOSPHATE AND CAL- 

 CIUM CARBONATE (BONE SALTS) PRECIPITATED 

 IN VARIOUS MEDIA, WITH APPLICATIONS TO 

 BONE FORMATION. 



JAMES CRAWFORD WATT, 



Anatomical Laboratory, University of Toronto. 



With Thirty-eight Figures in Three Plates. 



Contents. 



Page 



Introduction 280 



Part 1. Microscopic Study of Precipitation: 



Technique , 282 



Reaction in aqueous solutions 283 



Precipitation of calcium phosphate 283 



Precipitation of calcium carbonate 284 



Precipitation in colloidal solutions 284 



Precipitation of calcium phosphate 285 



Precipitation of calcium carbonate 285 



Influence of other agents on the precipitate 288 



Influence of different ions 290 



Precipitation in blood serum 291 



Precipitation in hyaline cartilage extract 291 



Influence of hydrogen ion concentration 292 



Disintegration of spherules 295 



Number of forms assumed by precipitate 297 



Part 2. Examination of Bone : 



Technique and material 298 



Results of examination of bone 3 00 



Part 3. Discussion : 



Theories regarding deposit of calcium salts 3 01 



Reversibility of calcium reaction 3°5 



Summary 3»7 



INTRODUCTION. 

 There are at present three main theories presented in explanation 

 of the manner in which the matrix of bone becomes impregnated 

 with the two inorganic salts, calcium phosphate and calcium car- 

 bonate. These views may be briefly summarized as follows : 



