104 BOTANICAL GAZETTE [AUG 
charge of the male nuclei, but may be absent later, which wo 
indicate that it too was ejected. In one instance (fig. 19) the nucleus _ 
was observed after ejectment. The male nuclei are of about 
same size and appearance, and leave the pollen tube at about 
same time. The nucleus which is to fuse with the endosperm nuc 
can be seen in various stages of its passage to the antipodal end 
-the embryo’sac. There is no evidence that either nucleus incre 
in size after leaving the pollen tube. The time of fusion with- 
polars may be either before or after their complete union with each 
other; in fig. 18 it is before. In jig. 18 the fusion of the two m 
nuclei with the egg and polar nuclei respectively is seen to be simul- 
taneous. After fertilization the egg secretes a wall about itself and 
rests for a time. a 
The occurrence of darkly-stained bodies so frequently seen 
_ presence in the pollen tube. 
THE SEED. 
The most noticeable change that results from fertilization is the 
extensive elongation of the entire ovule. Part of the growth is 
of the canal formed by the outer integument (fig. 22). A sim 
elongation of the outer integument was observed in Puya chi 
by Hormeister (4). | 
Accompanying the growth of the embryo sac is the develop nen 
of the endosperm. It begins to form at once after fertilization, 2™ 
the nuclei resulting from the first divisions of the endosperm nuclet 
take position at either end of the sac, leaving, however, a few to f 
tae parietal layer between. At the antipodal end, cell for 
with walls begins at once, and a number of large cells form bie 
which stands out conspicuously in the cavity of the sac, which oN" 
wise contains only a few free endosperm nuclei. At first this issue 
