a 
19044] COULTER & CHRYSLER—REGENERATION IN ZAMIA 
457 
of a seedling was also observed (fig. 6). The photograph shows a 
leaf arising from a bud on one side of the hypocotyl; another bud, 
younger than the one shown, is present on the opposite side. Ana- 
tains a_ strong 
present just be- 
neath the surface 
of the hypocotyl, 
and around the 
bud this layer 
bends outward 
to form a sort 
of collar (jig. 7). 
The tissues of the 
bud show no evi- 
dence of break- 
ing through the 
or <. cortex, as is the 
Fic.5.—Vertical | case with lateral 
ection through the roots; hence it is 
probable that 
Periderm; 7, root;7, growth of the 
vascular _cylinder. 
X 2. 
shoot started in 
the phellogen. 
Whether the bend of the hypocotyl indi- 
“ites some slight injury or not cannot be 
swered, at least there is no direct evi- 
dence of an injury of any kind. 
hat a so-called polarity does not 
he in this case the nature of the 
% cture produced at each end of the 
Utilated stem, seems to be indicated 
Y such a case as that represented by 
i 
& 8, in which two new shoots and a 
to9 ei 
3 pare arising from one end and a shoot 
tomical examination showed that the bud con- 
vascular strand that runs 
straight inwards to join one of the bundles 
of the hypocotyl. 
A layer of periderm is 
i lad 
ritalin ee 
Fic. 6.—Seedling showing 
production of shoot from the hypo- 
cotyl. 
