60 BOTANICAL GAZETTE [TANUARY 
cell at a point where the investment formed by the covering hyphae 
is incomplete. Secondly, the fact that, in very thick sections, one 
sometimes finds that the invaginated cavity of the central cell is 
connected with the outside by a comparatively large, broad opening, 
where we might naturally expect a small, narrow one, since the 
ascogenous hyphae are relatively small. The large opening suggests 
. the pushing in of a larger body, possibly the fertilized ‘trichogyne”’ 
cell above mentioned. Lastly, the frequency of occurrence in early 
stages of a comparatively large, deeply staining cell, lying to one 
side of the swollen central cell and not yet pressed into it, indicates 
that this deeply staining body is not a hypha with the origin BARKER 
- attributes to it, but is instead the cell above mentioned. Should 
this prove true, then the fertilized ascogonial cell is in reality the end 
cell, while the enormously swollen penultimate cell performs a sort 
of “nurse-cell” function, ultimately becoming entirely displaced, 
its contents digested and absorbed, by the ascogenous hyphae develop- 
ing within it. 
That the structures growing within the cavity in the central cell 
are segments of ascogenous hyphae, I have no doubt; therein I agree 
perfectly with BARKER. For one may see in sections of almost 
every immature fructification young segments which are elongated 
and twisted and hypha-like, and which sometimes show evidences 
of dividing by fission, as is pointed out by IKENOo. Later, these 
segments become rounded off and vacuoles appear in them; still 
further development results in the formation of their eight spores. 
The Monascus species which I have used was first sent from Java 
some years ago by D. G. FarrcHILp to ERwin F. SmitH and was 
regarded by the latter as M. purpureus Went. Professor HARPER, 
who in turn received some material from Dr. SmirH, has turned the 
fungus over to me for examination. So far as I am able to judge 
from gross measurements, and from a careful comparison of figures, 
this form agrees with BARKER’s description of his Monascus, and as 
well with the accounts of WENT, UyEpDA, and others, so that my 
opinion is that IkENo and Barker worked with similar forms. I 
find therefore in the main a confirmation of the conclusions of BARKER, — ; 
as well as ory rei gauges of the oe of IKENO: 
MAbpIson, 
