94 BOTANICAL GAZETTE [FEBRUARY 
gous to BUCHNER’s zymase, and like it glycolytic. This enzyme he 
reports in leaves and roots of beet, tubers of potato, seeds, seedlings, 
and young plants of pea, seedlings of barley, and entire plants of 
Paris quadrijolia. Confirmatory results have (naturally enough) 
been obtained by several students or assistants who have evidently 
been engaged upon portions of the problem under the guidance of 
STOKLASA. It is only fair to say that Mazé has strongly criticised — 
Stoxiasa’s methods from the bacteriological side, and declares 
himself unable to secure like fermentation under aseptic conditions ;"* 
though Sroxiasa claims to have guarded carefully against infection 
and to have rejected contaminated cultures. Independently, Mazé 
has found what he calls zymase, in connection with pea seedlings, 
Aspergillus and Eurotiopsis. He declares it “‘an enzyme normal to 
all plants, arising like all other enzymes during vegetative (aerobic) 
life.’ In the higher plants, however, and in most fungi it “is oxi 
dized with the greatest ease, so that one never finds more than a 
trace of it.” 
Mazé and STox.asa interpret their results somewhat differently, 
Mazé holding the process of fermentation to be a nutritive one," 
sugar being only assimilable when fermented and the nascent alcohol 
thus made available, while Sroxiasa believes fermentation to be 
merely anaerobic respiration and essentially a process for the imme- 
diate release of energy. 
Confirmation comes also from another source, for GopLEWSKI,"* _ 
working with lupines, finds similar products, and concludes that th 
“anaerobic respiration is identical with alcoholic fermentation, of 
at least in essence dependent on it.” 
Moreover, sgenekion nist = Maxtmow"® wets found 
Aspergillus an Tes i 
7 = yme and is responsible 
*4 Various papers in Annales Inst. Pasteur 18: 1903. 
_ 4s IWANOWSKY in 1894 propounded the theory that alcoholic fermentation 15 4 
pathological case in the nutrition of yeast, called forth by the abnormal com mposition. 
of the nutritive medium. 
os as — Ze Kennt. der — Evnases Bull. Acad. Sci. Craco’ 
(1904: 115-158. also his earlier paper with Porzentusz. Bull. cit. kot bs 
37 Ueber Pha der Scien Ber. Deutsch. Bot. Gesells. 
| 207-215. 1904. 
ca ar: gi itber die auiteek Ber. Deutsch. Bot. Gesells. 22: 225-235- 
