170 BOTANICAL GAZETTE [MARCH 
to the surface of the female nucleus, slips from its own nucleus, and 
was observed extending over fully two-thirds of the female nucleus 
(fig. 24). This behavior of the male cytoplasm has been observed 
by CoKER (3) in Taxodium and by Miss RoBERTSON (g) in Torreya 
californica. The male cytoplasm of Torreya taxijolia 1s sharply 
differentiated by staining from the cytoplasm of the egg, and undoubt- 
edly completely invests the fusion nucleus. The appearance of a 
similar mass of cytoplasm investing the free nuclei of the first division 
(fig. 25), and continued in the second division in connection with 
wall-formation (fig. 26) suggests the possibility that the male cyto- 
plasm may remain differentiated through more than one cell genera- 
tion. Near the neck end of the archegonium nuclei are evident, 
which seem to be the stalk and tube nuclei and the other male nucleus 
with its investing cytoplasm (fig. 24). 
EMBRYO. 
Soon after fertilization the first division of the egg nucleus was 
observed (fig. 25), and almost immediately the second division fol- 
lows, giving rise to four large free nuclei almost filling the egg (7g: 
26), one nucleus in the base of the egg, the other three in a plane 
above. At this time wall-formation occurs, the cytoplasmic radiations 
which precede it being very evident (fig. 26). Two weeks later the 
egg is completely filled with a proembryo consisting of twelve to 
eighteen cells (fig. 27). This complete filling of the egg by the pro- 
embryo is remarkable among Coniferales, having recently been 
observed also by Lawson (4) in Sequoia, but as yet not recorded 
in other genera. In Torreya, at least, this fact seems to be related 
tothe relatively small size of the egg, the very large nuclei, and the 
early appearance of walls. 
The cells of the proembryo at this early stage are distinctly in three 
tiers; that nearest the neck of the archegonium comprising five or SIX 
cells and forming the primary suspensor tier; the middle tier, Co™ 
prising five or six cells and forming the secondary suspensor tier; and 
the lowest consisting of a single cell which ultimately contributes to 
suspensor-formation and forms the embryo. The inequality in the 
number of cells entering into the tiers seems to be characteristic of 
Taxaceae. In Podocarpus CoKER (2) found the three tiers made up 
