1905] DEAN—PROTEOLYTIC ENZYMES 323 
of the negative results by this method has been called in question 
by FRANKFURT.° NEUMEISTER’S results may be summarized as 
follows: 
POSITIVE RESULTS NEGATIVE RESULTS 
Barley seedlings—sprouts 3°™ long. Barley seeds. 
Barley seedlings—sprouts 5 °™ long. Barley seedlings—sprouts 0.5 to 1°™. 
Barley seedlings—sprouts 15-20°™ long. 
Poppy seedlings. Poppy seeds. 
Beet seedlings. Beet seeds. 
Corn seedlings—sprouts 10 °™ long. Corn seeds. 
Corn seedlings—sprouts 16°™ long. . Lupin seeds. 
Wheat seedlings—sprouts 17°™ long. Lupin seedlings—sprouts 3°™. 
Lupin seedlings—sprouts 12°™. 
‘ Lupin seedlings—sprouts 20°™. 
Vicia see 
Vicia seedlings. 
ea seeds. 
Oat seedlings. 
_ A number of different investigators have worked on malt; the 
evidence shows that the germinating barley contains a proteolytic 
*nzyme. WErss’ thinks there are two, a peptic and a tryptic enzyme, 
. view which is supported by FERNBACH and Husert,® but ques- 
tioned by Vings. 
In the course of the extensive studies of Fermi and BuscaLIont® 
on proteases in plants, they tested a number of seeds and found 
that the ripe seeds of several plants contain an enzyme capable 
of liquefying gelatin. They likewise found that the germinating 
Seeds of some plants contained such enzymes, but in other cases 
no evidence of their presence could be found. 
BurKEwirscu'® made a careful study of the germinating seeds 
of two lupins, Z. angustijolius and L. luteus, and a somewhat shorter 
° FraNxkrurr, Landw. Versuchsstat. 47:466. 1896. 
7 WEIss, Zeitschr. fiir Physiol. Chem. 31:79. 
8 FERNBACH and HuBeErt, Compt. Rend. 130:1783.- 
® FER and Buscatront, Centralbl. Bact., etc. II. 5:24. 1899- 
*° BUTKEwrrtscu, Zeitschr. Physiol.-Chem. 32:1. 1got. 
