1905] CALDWELL—ACTION OF BROMELIN 413 
1o°* m/10000 Ba(NO,),. To each tube was then added 1%™ egg- 
albumen previously boiled and granulated, and the tubes were then 
placed in the water-bath at 40° C. for 24 hours. They were then 
removed, filtered to remove undissolved albumen, and tested. 
Tests were made for peptones, leucin, and tyrosin by all the 
standard tests. The biuret reaction after precipitation of the albu- 
moses, and the tryptophan reaction described by ViNEs (25, @) were 
OPTIMUM ACID MEDIUM m/32 HCl 
OPTIMUM ALKALINE MEDIUM 
m/ OH 
32 Na 
SALT | Dilution of molecular solution | Diluti f molecular solution 
| Inhibiting Allowing | Inhibiting | Allowing 
ea Si 
| eee 110,000 130,000 80,000 100,000 
PO Ni race ccd a's a wey 80,000 110,000 80,000 96,000 
ie ae 60,000 90,000 45,000 60,000 
fe ne 8 000 70,000 40,000 54,000 
EDs erica ¢ 55 Ks 75,000 90,000 64,000 80,000 
3) 65,000 85,000 45,000 60,000 
te 40,000 60,000 36,000 56,000 
pee ee ae 37,500 56,000 0,000 56,000 
lt (0) oe 28,000 32,000 16,000 22,000 
oS ae ne 16,750 23,000 14,000 17,000 
nl in 10,000 13,000 ,000 8, 
Se ae 8,000 12,000 6,000 (i Shee 
CAN Oa snd ca ,0 ,600 5,000 7,000 
RN eek nck ew 24 4,800 6,650 4,750 5,650 
Od 045 Ge ss 4,800 6,400 3,000 
Sheree sk era es 500 5500 O00 3,000 
LiNO, at itera ete cp ga : 1,200 
PAPO viveacc. ... 750 1,050 ° 
3 2 Se 512 1,024 500 
is Shas a eae 1,600 2,000 1,100 1,675 
oa goo 1,200 72 6 
a a een 750 1,100 650 1,050 
a eee 800 1,000 600 
MalN Os). ces os 650 | 1,000 3 512 
oS NO oe 00 | 1,000 375 512 
ae 100 | 160 60 120 
RG ee Sits a wes 100 200 125 575 
Ais 256 512 256 432 
PaO... a. 400 | 512 256 432 
15 ie Sa a 320 | 480 275 420 
found to be most delicate, HorrMANN’s and SCHERER’S tests being 
used as confirmatory. The unequal delicacy of the reactions employed 
made it necessary to adopt a rule to secure uniformity in results. 
Hence only such concentrations were considered inhibiting as gave 
ho results with any test, while the only strengths tabulated as allowing 
