1889.] of the Injection of Ferments. 17 



Rabbit 12 died 66 hours after its inoculation with anthrax. 

 The anthrax bacilli in the lung, spleen, and lymph gland near 

 seat of inoculation, instead of appearing in the form of short 

 rods characteristic of virulent anthrax, were arranged for the 

 most part m long chains as is usual with attenuated virus. The 

 chains consisted of as many as 12 and sometimes even more 

 joints. Two mice were inoculated from the heart-blood of this 

 rabbit, and died of anthrax after the rather unusually long period 

 of 60 hours. No. 11 died only 13 days after its inoculation. It 

 had slight diarrhoea for some days before its death. Its spleen 

 contained but few bacilli arranged in chains generally of six or 

 seven but sometimes of as many as fifteen joints. 



Three days after the inoculation of these rabbits another rabbit, 

 22, was inoculated from the same culture. The next day three cc. 

 of one per cent, pepsin was injected intravenously. The rabbit 

 died six days afterwards and its spleen shewed very few bacilli 

 all arranged in chains. 



Experiment 2. Three rabbits were inoculated with virulent 

 anthrax. Two days afterwards only one was still alive. It was 

 treated with four cc. of one per cent, trypsin injected into its ear 

 vein. It died four days after its anthrax inoculation. The spleen 

 contained numerous bacilli, which were for the most part arranged 

 in chains, one of which contained as many as 24 joints. These 

 chains shewed signs of degeneration, in that they stained very 

 irregularly. In the same chain some joints were colourless while 

 others were deeply tinted. The spleen of this rabbit was par- 

 ticularly large, and a great many of the cells contained more 

 than one nucleus, while other nuclei had a dotted aspect. Ap- 

 parently these appearances indicate an increased rate of nuclear 

 division*. 



Experiment 3. Professor Koch kindly inoculated for me five 

 rabbits, Nos. 26 to 30, each with a large quantity of anthrax 

 spores suspended in a normal salt solution. No. 29 served as 

 control and died in 36 hours. I injected three to four cc. of one 

 per cent, trypsin into the ear vein of Nos. 26, 27 and 28 directly 

 afterwards. No. 26 died in the same time as the control. None 

 of the bacilli were in very long chains, none of more than six 

 joints were seen, while most of the bacilli were exceptionally 

 short. This fact and also the way in which they were arranged 

 on the slide suggested to me that the bacilli had at first grown in 

 longer chains, but that at a later period (perhaps when the effect 

 of the trypsin had passed away) they had broken up into separate 

 segments. 



* My observations were all made on fresh preparations of the spleen pulp, to 

 which some dilute aqueons solution of methyl blue was generally added. 



VOL. VII. PT. 1. 2 



