18 Mr E. H. HanJcin, On a new result [Nov. 11, 



No. 27 died in about 50 hours. The bacilli in the spleen 

 were rather longer than usual. 



No. 28 died in 21| hours. Unfortunately I have mislaid my 

 notes of its post mortem appearances. 



No. 30 had four cc. of one per cent, trypsin injected intra- 

 venously the day after it was inoculated with anthrax. It died 

 in 36 hours and the spleen shewed the bacilli arranged in long 

 chains. 



Experiment 4. Five rabbits, Nos. 34 to 38, were as before in- 

 oculated by Professor Koch with anthrax spores. Directly after- 

 wards I administered intravenously from one to 3^- cc. of 05 per 

 cent, solution of trypsin. 



No. 38 was control and died between 50 and 60 hours after 

 inoculation. 



No. 34 weighed 1500 grammes and had one cc. of the above 

 solution of trypsin. It died 51 hours after inoculation. Some of 

 the bacilli were isolated, but many were in chains of more than 

 12 members. 



No. 35 weighed 1507 grammes and had 3^ cc. of the "05 per 

 cent, trypsin solution. It died in 60 hours, and the bacilli were 

 arranged in chains. No. 36 died after 36 hours*. The spleen 

 contained very few bacilli and of these some w r ere arranged in 

 chains. 



No. 37 weighed 1865 grammes. It had 2| cc. of the above 

 solution of trypsin. It was very ill for some days, but at last 

 recovered and is now alive and well nearly three months after the 

 operation. The temperature record of this unfortunately unique 

 case is very interesting. The day after inoculation the temperature 

 was 37°"4 Centigrade, i.e. 2*4 degrees below the normal tempera- 

 ture of a rabbit. It remained at approximately this low figure for 

 some days, shewing a very gradual rise, and only on the sixth 

 day after inoculation had it reached 38°. From this point it 

 rapidly rose till on the 11th day after inoculation it was 40°'l. 

 On the 12th day it stood at 40 o, 05, when observation of its tem- 

 perature was discontinued. Another interesting point about the 

 case was the appearance of pus at the seat of inoculation. On 

 the 8th day after the experiment began, a small hard tumour 

 about half-an-inch in diameter was found at the seat of inocu- 

 lation. On the 13th day a second larger tumour appeared in 

 front of the former. This gradually increased in size and was 

 found to contain caseating pus. About a week later, no further 

 increase in size could be noted. The animal appeared to be ema- 



* I noticed while this rabbit was being inoculated that it had a cough. This 

 point is worth noting, as I have usually observed that a lung disorder increases 

 susceptibility to anthrax. 



