1892.] Mr Shore, On Intravascular Clotting. 3U9 



extracts and fluids he injected are all artificial, and none, not even 

 the expressed juice from the lymphatic glands, can be considered 

 as normally present in the body in exactly the form in which he 

 injected them. It was therefore of the greatest importance from 

 his point of view to show that the lymph as it is normally passing 

 to the blood carries with it the very substances which the arti- 

 ficial extracts contain, viz. tissue fibrinogens, and that the lymph 

 itself when injected in the same way as the extracts, does by 

 virtue of these bodies produce intravascular clotting. The present 

 paper is a preliminary account of a few experiments I have so far 

 made which supplement and continue Wooldridge's work on this 

 point. The observations were made during the continuation of 

 my work on the action of peptone on the clotting of Lymph and 

 Blood 1 . It was shown by Heidenhain' 2 that when peptone is 

 injected into the blood, the flow of lymph is much increased and 

 the percentage of proteids in it increased. It was possible then, 

 that the blood had lost to the lymph certain proteids necessary to 

 clotting, and that the cause of the loss of clotting power was to be 

 sought by studying the relations of the lymph to the blood. The 

 addition of peptone lymph to peptone blood out of the body did 

 not lead to clotting. I then turned to the intravascular injection 

 of lymph, and first of all to normal lymph. 



The lymph was collected from the thoracic duct of a dog in 

 the ordinary way. In the first experiment 12 c.c. of lymph could 

 be collected before it clotted. This was quickly injected into the 

 jugular vein of a rabbit, there was some rapid respiration, and in 

 2 — 3 minutes the animal was dead. The heart, and all the 

 arteries and veins, even their small branches, as far as traced, con- 

 tained only clotted blood. The clotting was complete so that long 

 " blood casts " could be drawn from the vessels. A smaller quan- 

 tity of the same lymph, 5 c.c, was then collected and injected 

 into another rabbit. In about one minute the animal was dead, 

 and general intravascular clotting was found as before. It was at 

 once considered possible that the difference in the species of the 

 two animals might be of great importance. Richet and Hericourt 3 

 found that 12 c.c. of defibrinated dog's blood was sufficient to kill 

 a rabbit, but the condition found after death was not one of 

 general intravascular clotting, as only a few small clots were 

 formed, although there was a profound influence on the red 

 corpuscles. I injected 15 c.c. of freshly drawn arterial blood from 

 the same dog without producing any effect on the clotting power 

 of the rabbit's blood, neither hastening or retarding clotting. 

 Lymph from the same dog was allowed to clot, and 15 c.c. of the 

 lymph serum, injected into a rabbit, also produced no effect. 



1 Journal of Physiology, xi. 561. " Pfliiger's Archiv, xlix. 209. 



3 Comptes rendus de la Soc. de Biol, cvn, 718 and ex. 1282. 



