1892.] in producing Intravascular Clotting. 311 



the amount collected increased. A precipitate was thus at once 

 formed, this was separated by centrifngalisation, and then twice 

 washed with water as Wooldridge directed. On the addition of a 

 small quantity of alkali, very dilute sodic hydrate, the precipitate 

 almost completely dissolves. (It consists partly of leucocytes 

 separated by the centrifuge.) 



The tissue-fibrinogen solution itself clotted in 2 — 5 minutes to 

 a firm jelly. If injected at once into a rabbit, the animal dies 

 almost immediately, and clots are found in the heart and in some 

 cases the portal system. This result was however not always 

 produced, sometimes the animal remained alive, but its blood was 

 found to have lost its clotting power. This is in full accord with 

 the observations of Wooldridge, with tissue-fibrin ogen obtained 

 from glands and testis. 



A substance, which produces intravascular clotting, may be 

 obtained from lymph by a method indicated by Alexander 

 Schmidt 1 . He found that an alcoholic extract of lymphatic 

 glands, of liver, and other tissues contained a substance which 

 excited clotting in horse's plasma. I allowed lymph to drop from 

 the thoracic duct directly into alcohol, the precipitate was then 

 shaken with the alcohol for several hours, and then the alcohol 

 was poured off and evaporated to dryness at 37°, and the residue 

 shaken up with a little water. A considerable portion dissolved, 

 and 4 c.c. of this, which formed a clear, slightly pinkish coloured 

 solution, was injected into the jugular vein of a rabbit. The 

 animal died at once, and the heart and large veins were full of 

 clotted blood. The alcoholic extract of blood treated in the same 

 way does not produce intravascular clotting. 



(3) On the fever produced by the Injection of sterilized Vibrio 

 Metschnikovi cultures into rabbits. By E. H. Hankin, B.A., 

 Fellow of St John's College, and A. A.' Kanthack (M.B. Lond.), 

 St John's College. 



It is well known that a fever can be produced in rabbits by 

 intravenous injection of the products of the growth of Vibrio 

 Metschnikovi. a microbe closely allied to the Cholera-germ. The 

 serum of rabbits possesses a certain power of killing the Vibrio 

 Metschnikovi, and we have attempted to find out whether this 

 ' bactericidal power' is increased during or after the fever produced 

 by the method above mentioned. We have also noted the numbers 

 of leucocytes present in the rabbit's blood before and after the 

 fever, in the hope of being able to establish some relation between 



1 Centralblatt fiir Physiologic, iv. 257. 



