92 



Scientific Proceedings, Royal Dublin Society. 

 Table VIII. 



That the activity of the invertase added has not been limited is clear ; 

 this, though demonstrating that the negative results obtained with the sap 

 are not associated with the production of substances of a toxic nature, yet 

 does not indicate whether adsorption of the leaf enzyme, either before or 

 after extraction of the sap, or its destruction is the cause. 



As the factors inhibiting the activity of certain peroxidases have been 

 removed h^ dialysing the sap (1), it was thought that similar treatiaient might 

 possibly restore the activity of the enzyme in the sap pressed from toluened 

 material. To 50 e.c. of the dialysed sap were added 2 gms. of sucrose; aii equal 

 weight of sucrose was also added to 25 c.c. of the same sap, which, however, 

 had been stored for three days while dialysis was in progress iii the first 

 sample. The results are shown in Table IX ; the results obtained with a 

 sample of the sucrose-sap (not dialysed) diluted with an equal volume of 

 water are also given. 



■ Table IX. 



The dialysis has evidently not been responsible for any increase in 

 hydrolysis ; it is possible that both enzyme and inhibitor diffused away. 



