156 



Scientific Proceedings, Royal Dublin Society. 



downwards. This was immersed in the solution in the calorimeter. By 

 means of a combined force and suction-pump, the enzyme solution was 

 forced out of the pipette, and then the pipette was washed out by drawing 

 in some of the mixed liquid and again expelling it. As this method was 



Fio. 1. 



unsuitable for use with our apparatus, owing to the narrow necks of the 

 vacuum flasks, we decided to enclose the enzyme solution in a capsule of 

 paraffined paper, attached to one of the glass tubes of the thermocouple, 

 a similar arrangement being used in the control flask (see fig. 1). These 



