160 



Scientific Troceedings^ Royal Dublin Society. 



The apparatus was then set up and left overnight in order that the 

 temperature inside the capsules might become the same as that of the 

 surrounding liquid. The next morning the deflections of the galvanometer 

 were observed at intervals, and, if constant, the capsules were ruptured and 

 the experiment started. The fact that the deflections are constant before the 

 commencement of the experiment is a proof that no leakage of the enzyme 

 solution has taken place. The deflections were observed at regular intervals 

 while the apparatus was continually shaken. When it was desired to stop the 



180 



160 



140 



IZO 



100 



O-^'c 



O-S-c 



a 80 



■Q-Z'a 



a 60 



40 



T!T^ 



20 



20 



30 



40 50 60 70 



'lime in minutes. 



Fin. 2. 



80 



90 



100 



no 



120 



inversion, the experimental flask was rapidly opened, and 10 c.e. of ammonium 

 hydrate run in. The exact time at which this took place was noted. 



The amount of sucrose inverted was determined by measuring the reducing 

 power of the solution according to Pavy's method. 



A curve showing the rise in temperature was plotted, and by extrapolating 

 this curve the exact temperature at the moment at which inversion was 

 stopped can be deduced. From this curve another ciirve corrected for 

 radiation losses was drawn, according to the method described by Hill (4). 



The curve obtained in Exp. No. 7 is reproduced in fig. 2. It will be 



