14 - 48 £&£& pTlinlte^deffnUe^ . I 1 "/*-* ««.l« were diminished 8nd none 



arrhythmia evidently of v*g«» «*«,„« i. cherac- «!!!„**" m ' totlc figures. Some lymphocytes had 



Un.tlc for turtles. In o\,r observations Py c "° tic "ucle,, which were not noted in the in- 



the R-R interval fluctuated between 20-49 sec. animals. 



The repetition of cardiac contractions by T h ■ •, 



10-12 beats per minute occurred under a greater hi-- I Gemmiferous tubules in the testes were 



stimulus (injection by a needle in the ga'sTroc- J'1 '" w^*"" {f ' 9 *' ^ l] ' ^ br0Wn 



nemius). Return of the pulse frequency to the u 9 accumulated ,n the interstitial tissue, 



original level occurred relatively quickly, in -V"! """^ on ly in individual cells in the 



about 1-2 minutes. X q y ' ,ntact animals. This pigment was also detected 



in many remaining germ cells, had a dark orange 



On the 21st day after termination of the *"»*»> uncolored preparations, gave a negative 



flight an EKG was recorded and some results of reaction on iron, and was colored moderately by 



this investigation are presented in Table 1 Sudan. Conglomerates of the pigment possessed 



and F, 9- 2. birefringence. The number of germ cells and the 



TAD , _ , „ concentration of RNA therein diminished consider- 



TABLE 1. CHARACTERISTICS OF CAROIAC ACTIVITY ly (F ' 9S " 6 » 7)- The SDG activity increased 



OF TURTLES WHO FLEW IN "ZOND-V somewhat, but the MAO and alpha-GFD activity 



J decreased. 



No. Animal Heart Contraction R-R lntepval . 



Number Frequency per („ sec . Drawing The seminal vesicles of the test turtles 



Mi -te Rate 15 mm " c 9 V?l ""'T* ^} ^ ' *•* lun,,n was filled 



1 22 Tes 28 The RNA concentration and ferment activity in the 



I Hl eSi t , 28 32-34 tntJrl W " ,C 1 leS WaS Chan 9 ed as compared with the 



3 49 Control 32 29-31 '"tact control animals exactly as in the testes 

 flying to J 



cosmodrome A diminution in the size of the hepato- 



4 47 Control 3 2 2-23 ^ tes and ih *>r nuclei was observed in the liver 

 ln ovarium the cell cytoplasm became basophile (Fig. 10) 



, . The °. ua "tity of brownish, big-clump pigment was 

 As is seen from the Table and the EKG. no '"creased at places where the endothelial- 

 substantial differences between the test and r * t ! C ' jlar cells accumulated, and dustlike granules 

 control were detected in a number of the in- »f triv.lent iron increased in the lumen of the 

 dices studied, (cardiac contraction frequency, b , le cap , liar ies. Ri bonucle ic ac id was conta i ned 

 K K 'ntervai; As regards the change in the '" the nucleoli ° f liver cells and in a small 

 individual EKG peaks, it is impossible to make quantity in the perinuclear zone. The RNA con- 

 any definite conclusions because of insufficient "Ration was negligible, but higher than in the 

 * n '"J act an "nals. The glycogen content (Figs. 11, 



12; in not only the hepatocytes but also in the 



in a macroscopic investigation of the peripheral blood cells was also elevated, princi- 



internal organs it was clear that the thick- P *}}* .' n tne leucocytes and in the free reticular 



ness ot the intestine diminished over the Cells ln tne lumens of the vessels. Fat vanished 



whole extent of the test and control turtles. * r ° m the "w Mil., while it appeared in a signi- 



Ine liver surface in the test animals was f,Cant amount ( Fl 9- 13) in the intact animals, 



intensely brown; in the intact turtles, it was '" the liver of the intact turtles there was 



colors'^ W ! S , intermedia *e between these J 1 "?** ""outcropping of formazane corresponding 



colors ,n the control animals. to the SDG activity, they appeared in some hepato- 



<;. ... ^ tes ln the test animals, in many Kupfer cells, 



Significant differences were observed in a th f VeSSel walls » and the bile ducts; the AP 



oroanTnlV'^ 05 "^ investi 9ation of the It tin* T * U ° el ' viied ' On the other hand, 



organs of the experimental and the intact the MA0 activity In the hepatocytes was clearly 



Z il S \ diam «ter of the intestine and P L eduCed < Fi 9- 14 )» a "d suppressed altogether in 



tne thickness of the muscle layer diminished the P* r 'Pheral blood elements. 

 in the test animals, the villi were shortened 



eoith\" S i 9 \ 3 '" The mitotic activity of the t 4L The V ° lume of cel1 nuclei of the epithelium 



epithelial crypts was suppressed. Certain epi- ° f the twlsted duct = was diminished in the kidneys, 



tnelial ceUs had a pycnotic nucleus (Fig. 4). S ° m f nUCle ' beCame P/cnot ic, the cell outlines 



'he cells of the stroma of the villi in the unclear, grains of brown pigment sometimes appeared 



cytoplasm and some epithelial cells contained '" the Cel1 Ooplasm, which gave no reaction 



clumps of brown pigment not discernable in the "*° n and W3S not c °lored by sudan, and dust- 



mtact animals. The content of the PAS - llke 9 ran ules of an iron-containing pigment 



Positive substances was diminished (Fig. O appeared. Moreover, RNA appeared more weakly in 



'ne number f beaker-like cells diminished the nucle °li ln the epithelium cells of many 



MAO J°h ^ in tHe depth of the crypt, the ! W '' Sted ducts> Tne SDG activity, and to a lesser 



(GFD) L\ lP ^glycerophosphate dehydrogenase l?™' the / P act i v i ty, was elevated, while the 



iwu; activity decreased. MA0 and alpha GFD activity was lowered. 



