8 Scientific Proceedings, Royal Dublin Society. 



destruction of the enzymes of the tissues. By our experiments it has been 

 shown that, by means of liquid air, sap containing enzymes may be extracted 

 from cells without serious alteration. This sap, frozen immediately after 

 extraction, might be evaporated to dryness as ice under reduced pressure, as 

 described by L. F. Shakell (5), and the resulting powder stored and added to 

 the food as desired, to replace the enzymes lost by sterilization. 



Bibliography. 



1. Dixon, H. H., and Atkins, W. R. G. : Osmotic Pressures in Plants. 



I. — Methods of Extracting Sap from Plant Organs. Scient. Proc. Roy. 

 Dubl. Soc, vol. xiii (N.S.), 1913, p. 422. 



2. GiGLioLi, J. : Delia probabile funzione degli olii essenziali e di altri 



prodotti volatili delle piante, quale causa di movimento dei sucehi nei 

 tessuti viventi. Eendic. Ace. Lineei, vol. xx, 2° sem. 1911, p. 349. 



3. Harden, A. : Alcoholic Fermentation. Longmans, Green, & Co., London, 



1911, p. 15. 



4. Lebedeff, a. : Extraction de la Zymase par simple Maceration. Ann. 



rinst. Pasteur, torn, xxxvi, 1912, p. 8. 



5. Maximow, N. a. : Ohemische Schutzmittel der Pflanzen gegen Erfrieren. 



Ber. der Deut. Bot. Gesellschaft. 1912, Bd. 30, Heft. 2, s. 52. 



6. Shakell, L. F. : An Improved Method of Desiccation, with some 



applications to Biological Problems. American Journal of Physiology, 

 vol. xxiv, 1909, p. 325. 



