188.3.] in parts of plants other than seeds. 90S 



In both these cases again I was unable to find any proteid 

 granules or crystalloids. Since crystalloids have been discovered 

 by Cohn in the tuber of the Potato, I hoped to find similar bodies 

 in Ranunculus Ficaria and Bunium Fleccuosiim, but was disap- 

 pointed ; but it must be mentioned the latter had already 

 germinated and produced a green leaf. 



Fourth, Bulbs : 



Scilla Nutans. 

 Tulipa Suaredens. 

 Galanthus Plicatus. 

 Galanthus Nivalis. 

 Alium Stramonium. 

 Hyacinth us Amethystinus. 

 Muscari Comosum. 

 Narcissus Poetic us. 

 Narcissus lncomparabilis. 

 Narcissus Pseudo Narcissus. 



In none of these, except Narcissus Poeticus, could I find 

 any proteid granules. But in a bulb of Narcissus Poeticus which 

 had been preserved in methylated spirit, in that part of the bulb 

 which represents the stem I found numerous granular be dies, more 

 or less spherical in fonn, which easily dissolved in potash solution, 

 were insoluble in ether, alcohol, acetic acid or in solutions of sodic 

 chloride, either when sections containing them were immersed 

 directly they were cut in ether, or when first placed directly 

 in absolute alcohol; they also stained orange yellow with 

 iodine. 



From these reactions I conclude they are granules of proteid, 

 and from their insolubility in solutions of sodic chloride or acetic 

 acid, that they are proteids of the albuminate type. The granules 

 are of relatively large size, and it appears that only one occurs in 

 each cell. They consist of an outer hyaline and an inner opaque 

 part, and it was this latter which dissolved in dilute potash. 



In section stained with haematoxylon, it is often possible to see 

 the nucleus stained and in its place, side by side with the proteid 

 granule. In order to assure myself that these granules had not 

 been formed in consequence of the action of the methylated spirit, 

 I examined fresh specimens, and found that the granules were 

 present. In the fresh state they appeared to be homogeneous, and 

 dissolved entirely in dilute potash, and stained rather more deeply 

 with iodine than those which had been kept in methylated spirit. 

 In order to see if similar proteid granules occurred in other bulbs, 

 I examined the bulbs of the plants mentioned in the list given 

 above, but I failed to find them. Unfortunately, owing to the fact 

 that the observations upon Narcissus Poeticus had been made late in 



24—2 



