— 42 Drs Fantham and Porter, Some Insect Flagellates 
feeding material contained pre-flagellate and a number of flagellate 
herpetomonads. Twelve hours after the infective feed, the mouse 
seemed ill, shivering, its coat staring, showing some difficulty in 
swallowing and passing much urine. It grew weaker, and died 
50 hours after the first and only infective feed. Examination of 
the blood 12 hours after infective feed, negative ; at 24 hours, free, 
rounded, non-flagellate (leishmaniform) elements seen; at 30 hours, 
a parasite within a leucocyte and a single flagellate were present’ 
in caudal blood. At autopsy the liver and spleen were hyper-' 
trophied, and kidneys, spleen and liver all very friable. Non- 
flagellate, leishmaniform bodies were found in the liver, spleen, | 
- kidney and lung smears, They were not numerous. A few) 
flagellates were found in liver smears, and in still fewer numbers 
in those of the spleen. The control mouse, fed on milk only, died | 
four days later. Its organs yielded no parasites on examination. 
Experiment 2 (A.P.). Young, wild mouse, J", weight 3°2 grams, 
was fed once with the teased intestines of three Nepa cinerea | 
infected with H. jaculum, and was afterwards fed on milk. The 
intestinal material contained mostly post-flagellate forms of H. | 
jaculum, together with some flagellates, among which dividing | 
forms occurred. Four hours after feeding, the blood contained 
a very few rounded, leishmaniform elements, while a flagellate was | 
found in the blood 24 hours after the infecting feed. At 30 hours - 
and 40 hours the same condition was found. At 40 hours the 
mouse became very weak, coat staring, eyes very dull. Some | 
shivering occurred. It died 70 hours after the infective feed. 
The body showed some emaciation. The liver was slightly | 
enlarged and contained a number of flagellates and a few non- — 
flagellate forms. Some dividing flagellates also occurred. Both | 
flagellates and non-flagellate forms were present in the spleen, — 
which weighed ‘03 gram and was friable, and also in the bone- ~ 
‘marrow, though in smaller numbers than in the liver. A very few 
parasites occurred in the lungs and heart blood. The other organs _ 
were negative. The control mouse was killed five days later, and 
examination of smears of its organs was entirely negative. 
Experiment 3 (A.P.). A young, wild mouse, $, weight 34 — 
grams, was inoculated intraperitoneally with material provided by — 
very finely teasing up the entire alimentary tracts of three Nepa 
cinerea, infected with Herpetomonas jaculum. ‘The mouse was 
afterwards fed on milk only. Three hours after moculation, one 
flagellate in metamorphosis to the oval, post-flagellate stage was 
found in the blood. At 12, 24 and 40 hours, a few leishmaniform 
parasites occurred in the blood. Fifty hours after inoculation the 
mouse seemed ill and died at 60 hours, The body was somewhat 
emaciated, the spleen and liver enlarged. Weight of spleen was 
‘04 gram. <A very slight peritoneal exudate was present. Both 
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