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140 Drs Fantham and Porter, Further Experimental | 
were negative. The frog died on the 54th day after inoculation. — 
At autopsy the animal appeared somewhat anaemic, and both 
flagellate and postflagellate herpetomonads were present in the 
liver. ; 
Experiment 5 (A.P.). A large male toad, Bufo vulgaris, was 
inoculated subcutaneously with the rectal contents of one Nepa 
cinerea, containing recently formed postilagellate stages of Herpeto- 
monas jaculum. <A slight swelling and reddening at the site of 
inoculation occurred on the second day, but disappeared on the 
third. Blood smears were taken daily. Six days after inoculation, 
a leishmaniform parasite was found in the blood, but blood smears — 
on subsequent dates were negative, except on the eleventh day, 
when leishmaniform and young flagellate parasites were seen. 
The toad died forty days after inoculation. At the autopsy, 
leishmaniform parasites were found in the liver. 
Eaperiment 6 (H.B.F.). A mature female toad, Bufo vulgaris, 
was inoculated intraperitoneally with the gut contents of one 
Nepa cinerea, containing young flagellate forms of Herpetomonas 
jaculum. No untoward symptom of any sort was noticed in this 
toad. Blood smears were made daily for a period of forty-five 
days, but all were negative. Further periodic examinations were 
made and were negative. The experimental toad and its control 
animal were killed at the end of eighty days. The internal organs 
of both toads showed no herpetomonad parasites. 
Experiment 7 (A.P.). A young male newt, Molge vulgaris, was 
fed with the mid-gut of a Nepa cinerea, containing a few flagellate 
forms of Herpetomonas jaculum. The newt became less active but 
otherwise showed little signs of illness. Blood examinations were 
made daily for six days, but no parasites were found therein. 
Unfortunately, the newt escaped in the laboratory, and when found 
three days later, was dead and too decomposed to allow of detec- 
tion of parasites. From the examination of the blood alone, there 
does not appear to have been an infection. 
Experiments with tadpoles and Crithidia gerridis are in 
progress. 
(c) Eaperiments with Reptilia. 
Experiment 8 (H.B.F.). A male lizard, Lacerta vivipara, was 
fed on the gut contents of two Gerris paludum, one containing 
preflagellates and the other mostly postflagellate forms of 
Crithidia gerridis. The number of parasites in the feeding 
material was small. Blood examinations were made. Five 
days after the infective feed, the blood was found to contain 
rounded leishmaniform elements, some in process of division. 
Dividing parasites occurred both free and within leucocytes. 
Similar intracellular and free forms were present in the blood 
