142 Drs Fantham and Porter, Further Experimental 
a few flagellates and some postflagellates of Herpetomonas stra-_ 
tiomyiae. Twenty-four hours after the infective feed, the mouse 
commenced to shiver. The shivering became violent and con- | 
tinued so until the animal died. Attacks of rigor occurred early 
each morning but passed away after a couple of hours. The mouse 
became emaciated and died five days after the infective feed. 
Examination of the blood of the mouse was made daily, but no 
parasites were found therein. Smears of the organs made at 
autopsy showed that infection had occurred. Leishmaniform 
elements, mostly free, occurred in the liver, lung and kidney, the 
liver showing the greatest number of parasites. Some of the 
herpetomonads in the liver were in the multiplicative phase, 
showing division of both nucleus and blepharoplast, as well as the 
CE i a — a 
commencement of cytoplasmic cleavage. Other parasites showed — 
an indication of encystment, forming postflagellates like those 
found in the faeces of insects. Occasionally, seemingly uninucleate 
elements were found, but on careful analyses, these were ascertained 
to be really binucleate, the blepharoplast being superimposed upon 
the nucleus. Fully developed flagellates were not found, but some 
in process of elongation were present. 
_ HKaperiment 13 (A.P.). An adult female mouse was fed with 
a number of body lice, Pediculus vestimenti, obtained from several 
sources. Some of these lice contained Herpetomonas pedicult. 
No untoward symptoms were noticed for some time, but during 
the latter part of its life, the mouse became emaciated and was — 
subject to attacks of shivering. It lived for 72 days. Blood 
smears were made daily. Oval, leishmaniform bodies were found 
on the 4th, 7th, llth, 19th, 24th, 29th, 32nd, 39th, 47th, 
56th and 69th days after feeding on the lice. Dividing forms 
were noticed on the 26th, 35th and 62nd days, elongating para- 
sites on the 29th, 37th, 42nd, 58th and 67th days, while mature 
flagellate herpetomonads were seen on the 30th, 44th, 64th and 
71st days after feeding. At autopsy, the body was found to be 
emaciated and the mouse weighed 2 grams less than its control, 
both being of equal weight, 11°7 grams, at the commencement of 
the experiment. The liver was pale and the spleen enlarged and 
brittle. Oval, leishmaniform parasites were present in the liver, 
spleen, bone-marrow and heart blood. EHlongating forms and a 
flagellate herpetomonad also occurred in the liver. 
Experiment 14 (A.P.). An adult female mouse was fed with 
part of the liver of the above-mentioned mouse, infected with 
Herpetomonas pedicult. It soon began to lose weight and became 
very weak. It died on the 15th day. At autopsy, leishmaniform 
and flagellate H. pedicult were found. in the organs, much as in 
the mouse from which it had been infected. The parasite is thus 
transmissible from one mammal to another. 
