20 BULLETIN: MUSEUM OF COMPARATIVE ZOOLOGY. 
C. MATERIAL AND METHODS. 
The adults of Anolis carolinensis were obtained from Jacksonville, 
Florida, and later from Colmesneil, Texas. They were sent alive by 
express, being received in good condition. After arriving they could 
be kept alive for any length of time by feeding with living flies. With 
such material constantly on hand, the use of the various special nerve 
methods was practicable. The methods of staining found best 
adapted to the purpose of the present study were Weigert’s and Vom 
Rath’s. The projection drawings of the peripheral distribution of the 
cranial nerves were made from a head prepared by the Vom Rath 
method, which was found the best for this material. 
The animals were killed with chloroform; the head, with the 
anterior part of the body as far back as the region of the fore legs, was 
removed and, after some of the tissue on the right side had been cut 
away so as to expose the brain and insure more rapid penetration, 
was put into a Vom Rath’s solution (formula for vertebrates). The 
length of time necessary in this fluid depends upon the requirements 
of decalcification, for the fluid serves the double purpose of decalecity- 
ing the bone and impregnating the medullary sheaths of the nerve 
fibers. I found that a week to ten days served the purpose well. 
After the required length of time the specimen was brought through 
the grades of alcohol and left in 80% alcohol long enough to remove 
as much of the picric acid as would readily come out. The after 
treatment with pyroligneous acid was not used. From 100% alcohol 
it was cleared in cedar oil, then passed into xylol and, finally, embedded 
in paraffin. For better embedding it was found advantageous to use 
the air pump, either while the object was in the melted paraffin or 
before, while in the oil. It was always found necessary to re-embed 
several times during the cutting of a complete series, as there were 
cavities not filled at first. This resulted at several places in the series 
in partial sections which, however, were numbered in sequence with 
the others. The series from which the large plots were made was cut 
transversely 10 micra thick. In place of artificial orientation lines 
the median plane was used for projection on the frontal plane, but for 
projection on the sagittal plane there was no natural line available 
for the whole distance. The border of the upper lip served for this 
purpose as far back as the corner of the mouth. From this pot 
caudad the orientation was determined from a comparison with a 
drawing of the lateral external view of the same head, and also with 
sagittal sections of heads of the same size. 
