044 Scientific Proceedings, Royal Dublin Society. 
The number of cubic centimetres of Standard Ammonia solution 
(1 cc. = 0°01 mg. NH) actually required in the different 
deternunations for :— 
ee re eee 
Time. Unoxidized Nitrogen as— Nien Nee am 
ae oe Tefen || MHselies, || MAsavico, ne 
Original Mixture, , 0 1°7/ 2-2 3°9 — — = 
After 24 hours (1 day), Loi 2-0 371 1109) 0°75 3°85 
op 8 oo (4b Cen), Dial 2:0 4+] 1-2 0°75 4°85 
HAGE var tlh dyes 3-0 12) e4-o elites, 0-94 | 5-14 
A UOCOURE Ss KOIIIN Fe58); 2°5 20 | 4:5 | 1:5 0-94 | 5-44 
R408 a eet(TS ey tay 2°5 2-7 | 52 | o-4 1:50 | 6-70 
i OM Ole AE 2-4 94 | 4:8 | 3:0 1:88 | 6:68 
Sei OMMOntAS ae 374 — — 15°5 9-70 — 
So ae 5-0 25) 7-5) | 17-0. | 10-62) elena 
BE Tie ye: 4-4 ae! — | 20-0 | 12-50 a 
At the end of twenty-seven days, the jar containing the mix- 
ture was left at ordinary atmospheric temperatures, and no longer 
maintained at 21°C. The mixture then contained no nitrites, but 
the nitrates had increased in amount. 
It was not again examined until an interval of about two 
months had elapsed (/.e. three months since the commencement of 
the experiment), when the nitrates were found to have increased 
very considerably, and a small quantity of nitrite had also been 
produced. The mixture was once more examined at the end of 
seven monthlis, and finally at the end of eleven months. 
The results of this series of experiments are, in one way, very 
puzzling, as the amount of total nitrogen was found to increase as 
the experiment progressed to an extent which cannot be accounted 
for by mere errors of experiment. 
At first we thought that this increase might be accounted for 
by the following two causes : first, the room in which the mixture 
of sewage and sea-water was kept, adjoined a laboratory in which 
