4 : AMERICAN FERN JOURNAL 
collected in Wisconsin, by E. P. Sheldon at Osceola 
by C. A. Davis at Argyle; and specimens of both 
forms collected near Peru, Nebraska, by R. Kent Beattie. 
In all these speci the differences noted in the writer’s 
earlier article are quite evident; but the sheets all bear 
the name of P. atropurpurea. 
When the earlier report was made, culture experiments 
were in progress to determine whether or not specific 
differences would appear in the gametophyte generations 
of the two forms. Shortly afterward the writer came 
to the State College, leaving most of the older culture 
material at Indiana University. At the present time 
it seems that there will not soon be opportunity to 
carry on the culture work to any considerable extent, 
and so, it may be advisable to report the findings to 
date. 
The prothallia of both forms are very irregular in 
form, and no clear distinction has been made on that 
basis. The cells of the prothallia of P. glabella are 
slightly the larger, enough larger so that the plants 
could usually be distinguished readily when examined 
microscopically. As reported by W. N. Steil (Bot. Gaz. 
52: 400-401, 1911) P. atropurpurea is very largely or 
entirely apogamous. The same condition holds in the 
case of P. glabella as found by the writer. A full account 
of the conditions in this form will appear at an early 
date. 
The cultures left at Indiana University have been 
cared for by Dr. D. M. Mottier, through whose kindness 
I am able to report that the apogamous sporophytes of 
P. glabella are strictly true to type. The difference is 
quite noticeable at early stages and there is not a grada- 
tion into the P. atropurpurea characteristics. This 
gives rise to an interesting question. In the case of 
these two apogamous ferns, showing well marked differ- 
ences of specific rank, is there any likelihood of inter- 
gradation or any possible question as to the distinetness i 
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