31 
Each series of three tubes was then treated in the same 
manner as described in the case of P. sulphureus, etc., and 
the following results obtained :— 
AcIp SOLUTION. 
(a) Tube with proteolytic activity gave - 240.0: = NH, 
(0) ” ” »” ad 2°77 c.C. ” ”? 
(c) >? no a3 7? +. 1'8 c.c. > 7? 
ALKALINE SOLUTION. 
(a) Tube with proteolytic activity gave POC > NA, 
(d) ” 2 ” od I’°9 c.C. ” ” 
(c) - no 55 5 , SSSR fe 
NORMAL SOLUTION. 
(a) Tube with proteolytic activity gave , 120ce, = NH, 
(0) 2? ” a) . £’5 c.C. ” ” 
(c) 2 NO r aE. ET ere 
These results show that in this case acid aids while alkali 
retards proteolyses. 
The several species of fungi mentioned at the beginning were 
examined in the following manner :— 
1. Auto-digestions. 
2. Preparation of enzyme. 
3. Direct examination of the juice of the fungi. 
1. The auto-digestions were made chiefly in order to have 
material to fall back upon for confirmation of results, and were 
carried out in the following way :— 
In the case of each fungus two equal quantities were weighed 
out, one portion was boiled for a few minutes, both lots were 
then ground up separately in a mortar with sand and an equal 
volume of water till a homogeneous mixture was obtained ; they 
were then bottled and shaken up with a little toluol. 
2. The preparation of the enzyme was carried out as already 
described. 
3. Whilst the above-mentioned preparations were laid aside 
to be used afterwards for a detailed examination of the nature 
of each enzyme, the proteolytic activity of each of the fifteen 
