35 
On analysing the above table, subtracting the figures in the 
third column from those in columns (1) and (2) and taking the 
average, the figures set out in the following table are obtained :— 
Fungus . : : » HGl Na,Cog H,O 
Agaricus aggregatus . 19°0 100 7°5 
Pezza sp. ‘ i ; . 150 55 555 
Agaricussp. A. . , . (54 25 20°0 
Agaricus arvensis ; : 727% 60 
Amanita vaginatus ‘ : exnk5 2°5 10’0 
Na. 2p ; ; : . 10°0 2°5 45 
Agaricus melleus : ; 2250 45 85 
Lactarius insulsus ; ‘ cas 2°5 8-0 
Russula cyanoxantha ; P fer & 2°5 155 
Agaricussp. B. . ; ‘ eithes 5'0 45 
Lactarius cilicioides : ‘ . &o 6°5 _ 
Agaricus sp.C. . ; ; « 3@ 2°0 — 
Polyporus betulinus i : yo 5S 30 6°5, 
Russula Clusu . ; ‘ _ oS 45 4°5 
Agaricus squarrosus : . ‘a 85 45) 
In studying the above table one observes— 
1. That proteolytic activity is present in every case. 
2. That the proteolytic activity in the acid medium is dis- 
tinctly highest in three, viz.:—Agaricus aggregatus, Peziza, 
and No. 27. 
3. That the proteolytic activity in the alkaline medium is 
distinctly highest in one, viz. :—Agaricus squarrosus. 
4. That the proteolytic activity in the media to which neither 
acid nor alkali were added is distinctly highest in six, viz. :— 
Agaricus A., Amanita vaginatus, Agaricus melleus, Lactarius 
insulsus, Russula cyanoxantha, and Polyporus betulinus. 
5. That proteolytic activity is greater in acid than in alkaline 
media in three, viz. :—Lactarius cilicioides, Agaricus C., and 
Agaricus arvensis. 
6. That proteolytic activity is increased by both acid and 
alkali in Agaricus aggregatus. 
7. That the proteolytic activity is practically the same in all 
three media in the case of Agaricus B. 
Having finished this experiment with the above members of 
the higher fungi, and made enzymatic preparations from several 
others which have yet to be examined, pure cultures were made 
of the following lower fungi and bacteria :— 
Mucor Mucedo, Mucor spinosus, Mucor racemosus, Asper- 
gillus niger, Aspergillus glaucus, Aspergillus oryz@, Momilia 
