36 
candidia, Chalara mycoderma, Oidium lactis, Cladosporium 
herbarium, Bacillus subtilis, Tyrothrix tenuis, Bacillus pro- 
digiosus, Streptothrix lactict acidt. 
After getting pure cultures of the above fungi, ten flasks 
containing sterile malt extract were inoculated with the ten 
orms. 
The pure cultures of the bacteria were introduced separately 
to flasks containing sterile glucose peptone (1 per cent. peptone, 
4 per cent. glucose). When the development had proceeded 
sufficiently far the fungi or bacteria were separated from the 
nutrient medium, and both organisms and media tested for 
proteolytic activity. 
In the case of the fungi the malt extract was filtered off and 
the mass of fungus mycelium washed a few times with distilled 
water. The fungus was then ground into a fine state with sand and 
water, and the extract filtered off. Twenty-five c.c. of the filtrate 
were put into each of three flasks containing ‘2 gm. protein. To 
one 5 c.c. of a 20 per cent. solution of tannic acid was at once 
added, and all three flasks incubated at 47° C. for five hours. 
After this time the other two flasks received 5 c.c. of tannic 
acid ; all three made up to 50 c.c. and filtered. 
enty-five c.c. of the filtrate were evaporated down with a 
little concentrated sulphuric acid till fumes of the latter came 
off, when other 20 c.c. were added, and the flasks heated till the 
contents were clear, when distillation was carried out in the way 
already mentioned. 
The malt extract was tested in the same manner as the 
mycelium extract, 25 c.c. being use 
So far the experiments with Aspergillus miger and Clado- 
sporium herbarium have been completed with the olewte 
results :— 
(Figures represent tenths of I c.c. deci-normal ammonia.) 
Result of digestion with— 
Extract from 
Medium. Fungus. 
(t) (2) (3) = (2) (2) (3) 
Ashergillus niger : 127/127 30 20. 202-36 
Cladosporium herbarium. ; 42 4I 21 18.517 270 
from which we get the following figures, ae average 
proteolytic activity in the medium and mycelium : 
Extract from 
Medium. Mycelium. 
Aspergillus niger : acy Io 
Cladosporium herbarium + 20°5 7°5 
