436 



Ich kann diese Arbeit nicht schließen, ohne meinem hochver- 

 ehrten Lehrer, Herrn Prof. Dr. F. E. Schulze, in dessen Institut die- 

 selbe gefertigt wurde, meinen ergebenen Dank abzustatten für die 

 mannigfachen, mir von ihm gegebenen Anregungen und das Interesse, 

 das er durch Gewährung der Hilfsmittel des Institutes für sie an den 

 Tag gelegt hat. 



IL Mittheilungen aus Museen, Instituten etc. 



1. Chloretone (Acetonchloroform): an Anaesthetic and Macerating Agent 



for Lower Animals. 



Von Harriet Randolph. 



eingeg. 21. Juni 1900. 



Acetonchloroform known commercially in America 1 as Chlore- 

 tone, whose hypnotic and anaesthetic qualities are being recognized 

 in medical practice 2 , may also prove valuable in the zoological laboratory. 



Chloretone is a white crystalline compound having a camphora- 

 ceous odour. Its action is supposed to be slightly preservative. It is 

 soluble in cold water in about the proportion of I : 150. 



By the use of Chloretone solutions of appropriate strength the 

 activity of rapidly moving microscopic animals can be reduced to the 

 desired extent. Such a degree of anaesthesia can be produced as to 

 make possible the fixation of some sensitive forms in a well-extended 

 condition. If, however, these are transferred from the Chloretone so- 

 lution to water again, an apparently complete recovery of the normal 

 condition takes place. This suggests that Chloretone may be used to 

 advantage in grafting and other experiments that require delicate 

 manipulation. 



In solutions too weak to produce anaesthesia the animals in every 

 case remained apparently in a normal condition for several days, until 

 removed from the solution. Strong solutions in some cases bring about 

 violent contraction followed by the disintegration of the animal. By 

 this method fine maceration preparations can be obtained. In Chlore- 

 tone solutions the transparency of such animals as Daphnia and Roti- 



1 A one per cent solution of acetonchloroform has been used for some years in 

 Europe as aneson. 



2 a) »Recent Advances in the treatment of Insomnia«. Reynold Hebb Nilcox 

 M. A., M. D., L. L. D. Medical News April 14th 1900. — b) »Chloretone: a New 

 Hypnotic and Anesthetic«. E. M. Houghton Ph. C, M. D. ; and T. B. Aldrich 

 Ph. D. Press of Parke Davis & Co., Detroit, Mich., U. S. A. 



