516 



saffranin, methyl green etc. In two places, viz. the intestine and the 

 testis, the homogeneous appearance of the spores is lost and various 

 stages in the formation of exceedingly minute germs which I have 

 interpreted as sporozoites, are found. Owing to the extremely small 

 size of the spores the process of sporozoite-formation is almost impos- 

 sible to follow and the following results may not be entirely free from 

 errors. The material of the spore segregates into a comparatively thick 

 shell about the periphery of the spore but within the spore membrane. 

 It then breaks or divides by multiple division into eight minute parts 

 all contained within the spore membrane which at this period for the 

 first time, can be distinctly seen (fig. IB). The sporozoites then leave 

 the spore, by what means I do not know, and, in the intestine are 

 lost sight of among the bacteria; in the testis they collect around the 

 various lobes as minute spherical granules which stain less intensely 

 than the original spores. The empty spore cases are frequently seen 

 with a rupture or a crack at some part through which the sporozoites 

 evidently made their exit (fig. 1 i?, C). 



The next stages of the young sporozoites rest in considerable 

 obscurity. Lost in the hordes of intestinal bacteria the naked germs 



Fig. 2. Development of the sporozoite in the lymph. Camera lucida X 2000. 



could not be followed. The mere fact that they are naked however, 

 makes it probable that they do not leave the intestine for an extra- 

 corporeal mode of life but that their further history takes place in the 

 epithelial cells and in the lymph spaces. The only positive evidence 

 that I can bring forth to show an intra-cellular stage is the fact that 

 minute granules, apparently foreign to the epithelial cells are fre- 

 quently found in the cytoplasm. While I cannot be certain that these 

 granules are young phases of the parasite, no such doubt rests upon 

 the small spherical bodies which were frequently found in consider- 

 able abundance in the lymph which surrounds the digestive tract. 

 Various stages were found here from small granules scarcely larger 

 than the youngest sporozoites, to the adult amoeboid organisms 25 

 to 30 microns in length. 



The various stages in the development of the sporozoite into the 

 adult organism can be seen at a glance in the accompanying figure 2, 



