(4^3) 



Such specimens can be sent through the mail or express and be 

 received in good condition, even after a long journey, if precaution 

 is taken to wrap them in waxed paper such as florists use. I have 

 received specimens from Europe in this way which were in a good 

 viable condition. Specimens which have reached maturity in 

 the field and have expanded after a rain and partially germinated 

 are never so desirable as those which have never been expanded 

 and gelatinized. Specimens which have been thoroughly dried 

 for herbarium purposes are useless for culture work. 



Author's Experimental Work 

 Five seasons 1 of culture work with the species of Gymnospo- 

 Tangium have been carried on as a part of the investigations sum- 

 marized in this paper. Out of a total of 33 species now recognized 

 in their telial phase, 26 have been available for the culture work. 

 Successful cultures were secured in 18 of the 26 species tested. Of 

 these, 9 were verifications of life-cycles previously ascertained and 

 9 gave aecial and telial connections never before recorded. In this 

 work 233 individual plant cultures were attempted and 25 species 

 of trial hosts were employed, belonging chiefly to the apple family, 

 Malaceae (i. e., Pomaceae). The following is a brief summary 

 of the results of the work during the different seasons: 



1906 1907 1908 1909 1910 Total 



Number of species of Gymno sporangium 



Number of plant cultures attempted.. 27 50 72 S l 33 233 



Number of species of trial hosts 



employed 10 13 16 17 13 25 



Summary of Original Cultures 



In order to bring together the scattered results pertaining to 



the cultures of Gymno sporangium, a table has been prepared giving 



a record of the first cultures of those species whose life-histories 



•The sixth season has been progressing as this paper has been going through 

 the press. Three new life-cycles have been worked out. See accompanying 



