(43) 
number of chromosomes in pure Oenothera Lamarckiana as 
14. The agreement of this number with that obtained by 
Gates in this country and with Geert in Holland, removes all 
reasonable doubt on this important point. 
The mechanical labor involved in the preparation of material 
(collecting, imbedding, sectioning, mounting, and staining’ for 
such cytological studies as this, and for other histological re- 
searches is very considerable and often burdensome to the 
investigator, and the need of a permanent expert technician, 
or preparator, competent to do this work, is felt by several 
members of the staff. Such assistance would mean the saving 
of valuable time, and would thus make possible the under- 
taking and completing of more extended research than can 
now be accomplished. 
A rearrangement of the herbaria at the Garden has neces- 
sitated the occupation of the room hitherto known as the Phys- 
iological Laboratory by the cases of the fungal herbarium. 
This deprives the laboratories of a room much used, and of 
special advantage in several ways on account of its skylight. 
This change also emphasizes the need of additional cases 
suitable for the storage of some of the larger pieces of glass- 
ware and apparatus. Physiological experiments would be 
greatly facilitated by the construction of a mezzanine floor 
under the skylight and above the herbarium-cases in the room 
above mentioned. 
The investigations undertaken during the year have not 
necessitated the purchase of any large pieces of apparatus. 
The equipment has been enlarged by the purchase of nu- 
merous smaller pieces to facilitate the researches in progress, 
and the appropriation not thus used has been expended for 
stains, reagents, and microscopical accessories. 
Early in the spring the organization of the Morphological 
Garden was assigned to me. Several of the old beds have 
seen enlarged, and five new beds started, in which are em- 
shasized the ecological features, or the relation and adaptation 
of plants and plant organs to the various factors of environ- 
nent. These five beds illustrate, respectively : 
