218 



SCIENCE 



[JST. S. Vol. XLIV. No. 1128 



per cent, of globulin was obtained by dialyzing the 

 extract. This globulin was composed of two pro- 

 teins which may be separated by fractional precipi- 

 tation with ammonium sulphate. These are desig- 

 nated globulin A and globulin B. Globulin A was 

 present in very small amount and gave the follow- 

 ing figures: C = 53.35, H = 6.95, N = 16.62, 

 S = 0.81, = 22.27. Globulin B, which was the 

 chief protein present, gave the following percent- 

 ages: = 53.21, H = 7.02, N = 16.77, S = 0.51, 

 O = 22.49. The nitrogen in globulin B was distri- 

 buted as follows: Humin nitrogen 0.30, amide ni- 

 trogen 1.40, basic nitrogen 3.17, non-basic nitrogen 

 11.53, total nitrogen 16.40. 



An albumin of the legumelin type was also ob- 

 tained from the Jack bean. This gave the follow- 

 ing figures: = 53.23, H = 6.99, N = 16.30, 

 S = 0.87, O = 22.61. The nitrogen was distributed 

 as follows: Humin nitrogen 0.23, amide nitrogen 

 1.16, basic nitrogen 3.73, non-basic nitrogen 11.18, 

 total nitrogen 16.30. 



On an Alcohol- Soluble Protein from Kafir-Corn, 

 Andropogon sorghum: Carl O. Johns and J. F. 

 Bkewstee. 



About three per cent, of an alcohol-soluble pro- 

 tein was obtained by extracting kafir-corn meal 

 with hot 70 per cent, alcohol. The purified protein 

 gave the following percentages: 



= 55.41, H = 7.25, N = 16.38, S = 0.62, 

 = 20.34 



The nitrogen distribution calculated from a Van 

 Slyke analysis was as follows: 



Humin nitrogen 0.17 



Amide nitrogen 3.46 



Basic nitrogen 1.04 



Non-basic nitrogen 11.97 



Total nitrogen 16.64 



The distribution of the basic nitrogen, calculated 

 to the per cent, of amino acids in the proteins, was 

 as follows: 



Arginin 1.58 



Lysin 0.90 



Cystm 0.78 



Histidin 1.00 



Tryptophan present. 



While this protein resembles zein from maize in its 

 ultimate composition, it differs from zein which is 

 lacking in lysin and tryptophan. Further investi- 

 gations are in progress. 

 A Synthesis of Tetracarbonimid: David E. Wor- 



RALL AND MARION K. McNaMARA. 



The oxidation of uric acid by hydrogen peroxide 

 in alkaline solution results in the formation of 



tetracarbonimid. This substance has been synthe- 

 sized in this laboratory by heating, in alcoholic 

 solutions, molecular amounts of carbonyl dimethan 

 and urea. The two substances slowly combine 

 with the elimination of two molecules of alcohol 



,NH.CO-;OC,H, HiH— N 



OC' | "+ | 



\\H-CO-:OC,H, H:H- 



^CO 



N/ 



/NH-CO-NH. 



= oc/ 



NCO + 2C 2 H 5 0H. 

 NH-CO-NH/ 



A Chemical and Bacteriological Study of some 



Non-Pathological Gastric Eesiduums: Chester 



C. Fowler, Max Levine and Sue B. More. 



The contents of forty fasting human stomachs 



free from gastric symptoms were examined for 



free and total acid, pepsin, trypsin and bile. The 



volumes and physical characteristics were noted 



and the number and kinds of organisms determined 



by plating on wort agar and plain and glucose 



agar. 



The stomachs fall into three groups: (a) prac- 

 tically sterile, (6) containing less than 2,000 or- 

 ganisms per c.c, (c) containing more than 4,000 

 per c.c. 



There were three main groups of yeasts, (1) 

 not producing gas from substance tested, (2) 

 forming gas from glucose, fructose and galactose, 

 (3) forming gas from these mono-saccharides and 

 maltose. 



Many of these yeasts formed aeetyl-methyl-ear- 

 binol (CH 3 CHOH.CO.CH„). 



A Study of Eighty Samples of Gastric Eesiduums 

 obtained from Apparently Normal Women: 

 Chester C. Fowler and Zelma Zentmire. 

 Sixty women were the subjects of this experi- 

 ment. Twenty-one submitted to the collection of 

 samples a second time; making a total of eighty- 

 one samples. 



The determinations made were: total and free 

 acid, pepsin and trypsin. 



The averages obtained were: volume 49.44 c.c, 

 total acid 30.31 c.c. (N/10 alkali to neutralize 

 100 c.c. of juice), free acid 15.63 c.c, pepsin 3.32, 

 and trypsin 5.22. 



A marked constancy in the residuum of the same 

 individual at different times was noted. In gen- 

 eral the results of Fowler, Eehfuss and Hawk ob- 

 tained on men at Philadelphia were confirmed. 



Charles L. Parsons, 



Secretary 

 (To be continued) 



