156 



SCIENCE 



[N. S. Vol. XXXVI. No. 918 



black, erumpent, produced at random mostly 

 near line of streak. 



Apple Fruit Agar. — Growth very abundant; 

 aerial mycelium growing in very dense, green- 

 ish gray patches in center and whitish around 

 edges of culture; pycnidia black, small, abun- 

 dant, produced mostly along line of streak. 



Synthetic Agar. — Growth very thick and 

 somewhat stromatic along middle; greenish- 

 black and white on surface, usually with the 

 white in the central part of the culture with a 

 greenish-black band around it. Surface be- 

 coming black all over with age. 



Microscopic Features. — Conidia, mycelium, 

 pycnidia typical. Large numbers of one- 

 celled, black chlamydospores produced on 

 mycelium by simple swelling and thickening 

 of certain cells. These chlamydospores have 

 germinated after six months' drying in the 

 laboratory and produced typical colonies of 

 the fungus again. These spores are thick- 

 walled and resistant and no doubt aid in tid- 

 ing the fungus over unfavorable conditions. 



STRAIN NO. 2 



Apple Leaf Agar. — Growth diffuse; my- 

 celium very light brown in mass; aerial my- 

 celium practically none; pycnidia extremely 

 abundant, produced usually over the whole 

 surface of the culture with a distinct concen- 

 tric ring formation even in tube cultures. 

 Sometimes the pycnidia are produced so thick 

 and close along the line of streak that they 

 form a well-marked black line. Conidia ooze 

 out in distinctly pink masses. This character 

 alone serves to distinguish No. 2 from the 

 others. This strain is a very prolific spore 

 producer. 



Apple Fruit Agar. — ^Diffuse; numerous 

 pycnidia mostly in a wide strip along line of 

 streak with a few scattered ones at base of 

 slant. Aerial hyphse short, gray all over the 

 surface of culture. Spore masses pink. 



Synthetic Agar. — Diffuse; pink; with long, 

 fluffy, pinkish-white aerial mycelium covering 

 surface of culture and growing up on sides of 

 tube. In some of the tubes this aerial my- 

 celium has a bright-green cast at apex and 



base of culture. Pink pycnidia very abundant 

 all over the surface and some even produced 

 up on the sides of the tube above the agar. A 

 decided tendency to concentric rings is noted. 

 Microscopic Features. — Conidia, mycelium, 

 pycnidia typical. No chlamydospores. 



STRAIN NO. 3 



Apple Leaf Agar. — Diffuse; mycelium dark 

 brown in mass; aerial hyphse gray, matted to- 

 gether, rather abundant over most of the sur- 

 face. Pycnidia abundant, very small, black, 

 erumpent, quite evenly distributed over the 

 surface of the culture with some tendency to 

 concentricity of arrangement. Agar turning 

 quite black throughout. 



Apple Fruit Agar. — Very diffuse but shal- 

 low. Surface covered all over with a dense 

 growth of long, greenish-gray aerial mycelium. 

 By holding to light the numerous black 

 pycnidia can be seen through the aerial my- 

 celium arranged in concentric rings. 



Synthetic Agar. — Growth abundant; sur- 

 face covered with a pink mycelial mass ; aerial 

 hyphfe very short, pink. Pycnidia inconspicu- 

 ous. 



Microscopic Features. — Same as ISTo. 2. 



STRAIN NO. 4 



Apple Leaf Agar. — Diffuse; mycelium in 

 mass very dark; aerial mycelium abundant, 

 gray, quite dense. Pycnidia minute, black, 

 abundant, evenly distributed, inconspicuous. 

 Distinct concentric rings have been noticed, 

 due to difference in color of different zones of 

 the mycelium. 



Apple Fruit Agar. — A very dense growth of 

 short, greenish-gray aerial mycelium forming 

 a mat over surface. Pycnidia very abundant, 

 minute, inconspicuous, black, evenly distrib- 

 uted. 



Synthetic Agar. — Dense stroma-like mass, 

 greenish-yellow on surface. In some tubes 

 the green is very pronounced around the 

 edges, while yellow predominates in the 

 center. Pycnidia inconspicuous. 



Microscopic Features. — Mycelium rather 

 larger than in other strains and noticeably 



