1921.] The Bighth Indian Science Congress. CCX Vil 
during the greater part of the year and the continuance of vaccine opera- 
tions all through the year have contributed to these poor resu ults. 
The Vaccinifer, 
‘alves of suitable size and good physique are grin to obtain to 
meet the large requirements and the absence of sufficient pasture in the 
premises of the institute has added to the difficulties of ec graces : 
bealthy and ‘oust set of calves required for the production of a pote 
virus 
The Maintenance of the Seed. 
‘The system of ing the vaccine virus from calf to calf with pre- 
setved zlycerinated ernulsion of the pulp, ends rg of virulence after 
or 6 passages. e system of direct transfer fie yielded, what appears 
to be satisfactory lg as judged by 16 moves from calf ts calf so far 
made. 1e maximum potency of the virus is reached in the cold weather 
at the end of 120 age in the hotter months, however, vesicles are 
found to mature at 96 hours sole even earlier, but these are inferior in 
quality to the vesicles efi 120 hou 
Grinding and mixing the Pulp. 
The use of mortars and pestles for i ay the pulp has not yielded 
satis nike A results. i hee tie ding machine (Chaly baus or 
Doering’s) is quite necessary to porn a seh: Ne of the pulp 
material aid its sito pt Beets tn the final produc 
The Choice of the Vehicle for the Vaccine Virus. 
King used lanoline and claimed for it the aavennner mh its being a 
capueal. aehicle without any antiseptic action 
ductor of heat and an —— —_ medium for ‘the veoh ae peat jaitieelion- 
tion of mage organ 
Other observers in Tada have found that lanoline is most unsul emt 
for the sees te for several cogent reasons, and t that glycerine is a mu 
more satisfactory vehicle. 
espinal Study of Chloroformed Glycerine Lymph, Unchloroformed 
Glycerine Lymph with and Lanolin e Lymph neleroree to their 
relative efficacy and keeping Propertt 
Chioroformed lage lymph was found to 0 oy xe all adverse 
influences of climate and to y} ield pig vee nt res ager ee gogo 
glycerine |! h npr h inferior to the 
properties rg iis as was found to be definitely iuperior to lanolinated 
Storage of the Lymph. 
periods was found to tell very 
walk on the potency of the virus as ce aled by rae rigat of Leacgreasieag 
during the period 1912-1920, rae — storage Ww 
2 
heat is the all important factor during t 
who bay yma Ps exposure tot ee vation of the vaccine after 
manufaetiire t till the time of despatch by ara at as low a temperatu 
