® 
1921.) The Eighth Indian Science Congress. cexix 
oe ek in ee of close attention to all the above details ee 
contin o give unsatisfactory results, the desirability =i = ing the 
vaccine y aopot toa “hill station, has to be seriously consider 
Phe influence of age and rit mabe on bacterial vac- 
cines.—By W. F. Harv K. R. K. Tvenear and 
S. R. CHRISTOPHERS. 
It is rather difficult to give a single quantitative aint to the 
effect of age and of temperature upon the bacterial antigen used in these 
insets As enka our poreest hs however, ‘Micke is no evidence on which to 
g : 
ae administer To cover all possibilities, and at the same time to 
sie on the side of ahs ety, it might be advisable to use the vaccines here 
dealt with wi ne some increase of dose rather than to reject them at some 
n _ er ich i i 
ou. 
experiments is ace wide range of variation in oe to inoculation of 
antigen exhibit ted by different adividuele among the experimental ani- 
mals, In the case of the Cholera antigen the measurable herronnig of the 
antigenic suspension fell somewhat rapidly from its va Psd baer 4 
tag bu at did not undergo any very rapid ¢ changes subse rit 
the case of th urbi apd raters 
out the lesa of trial whether for antigen ke ept om temperature 
or kept a 37°C. It does not follow of course, ay wiih autolysis, anti- 
geni 'y likewise to 
eep antigen ion, r 
storage temperature. They may, so far as turbidity is a gauge of their 
condition be allowed to reach : stable —— see they will retain that 
position for 12 m ioe at least, at room temperatu 
In the ments shown for the 8th and 19th rick 2 iow te 
there is evidence of actual increase in antigenic power. refrain from 
ing 7 nelusi i wo resu It n 
es do 
Potency as judg ed by agglutination response, within twelve mont 
any temperature to which they are likely to be exposed during that 
sited 
The production of B. influenzae vaccine on a large scale. — 
ae R. H. MaLone 
! method Seaetan in this so the organisms are grown in 
special uid n medium in whic a rapid sedimentation occurs. The pillaehg 
tant fluid is removed by means of @ su uetion pump, and the sediment 
oa 0 tg in saline and (ar as a vaccin 
culture iadiane employed is Doula mutton broth, P,,7'2 to 
7-4, containing 0°5 per cent heated pigeon blood and 0°5 per cent glooose 
S$ proc = results in a saving of tim 2 ekoor, « nd material, and 
allows of a more rapid production of influ en vaccine on a large scale 
than the methods sencnsinty employed. 
— viability of bacterial cultures.—By Nano Lat. 
Sealing of cultures is an important factor in their preservation. 
2 Haemophilic organisms such as the Meningococcus, Gonococcus 
